The failure of β cells to secrete sufficient amounts of insulin is a key feature of diabetes mellitus. Each β cell secretes only a small amount of insulin upon stimulation in a highly regulated fashion: young insulin is preferentially released, whereas old insulin is mainly degraded within the β cell. How this process is regulated in vivo and likely altered in diabetes is currently unknown. We present here a transgenic pig model that allows the in vivo fluorescent labeling of age-distinct insulin secretory granule pools, hence providing a close-to-life readout of insulin turnover. This will enable the study of alterations in β cell function in an animal model close to humans.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:76519 |
| Date | 09 November 2021 |
| Creators | Kemter, Elisabeth, Müller, Andreas, Neukam, Martin, Ivanova, Anna, Klymiuk, Nikolai, Renner, Simone, Yang, Kaiyuan, Broichhagen, Johannes, Kurome, Mayuko, Zakhartchenko, Varlie, Kessler, Barbara, Knoch, Klaus-Peter, Bickle, Marc, Ludwig, Barbara, Johnsson, Kai, Lickert, Heiko, Kurth, Thomas, Wolf, Eckhard, Solimena, Michele |
| Contributors | National Academy of Sciences of the United States of America |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/acceptedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 0027-8424, 1091-6490, https://doi.org/10.1073/pnas.2107665118 |
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