The objective of this study was to examine the inhibition of protein synthesis in murine P388 cells by sodium cyanate (NaOCN). The characterization of amino acid transport systems present in P388 cells provided a basis for some of these studies. NaOCN had no effect on the kinetics of amino acid transport systems L, A, ASC, N, or y$ sp+.$ The decrease in protein synthesis seen after NaOCN treatment was not secondary to alterations in amino acid metabolism or changes in nucleotide pools. Significant reductions in DNA and RNA synthesis were observed in P388 cells from NaOCN pretreated mice. No effect with NaOCN was observed on total cellular RNA. NaOCN does not appear to affect the P388 cell ribosomal machinery, tRNA or protein synthesis elongation processes. The formation of the 48 S initiation complex was significantly inhibited by NaOCN. These results suggest that the decrease in protein synthesis observed with NaOCN in P388 cells is due to alterations in mRNA synthesis and/or the inhibition of the early stages of protein synthesis initiation.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.75367 |
| Date | January 1987 |
| Creators | Lazarus, Philip. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Division of Experimental Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000419821, proquestno: AAINL38342, Theses scanned by UMI/ProQuest. |
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