In the rat, basement membranes are thin, resilient layers which support epithelia and endothelia and form a covering for muscle fibers, fat cells and the nervous system. By immunostaining for light microscopy with peroxidase methods, all examined basement membranes were shown to contain type IV and V collagen, laminin, heparan sulfate proteoglycan and fibronectin. Immunostaining for electron microscopy revealed that the five substances are localized in the basal lamina part of the basement membrane. / Reichert's membrane, a multilayered basement membrane, was shown by immunostaining to contain type IV collagen and laminin. To examine the origin of these two substances, the endodermal cells associated with Reichert's membrane were immunostained and displayed reactivity in rough endoplasmic reticulum, Golgi saccules and secretory granules, a result suggesting that the two substances are secreted along the usual pathway of protein synthesis. The endothelial cells in the incisor tooth showed similar cytoplasmic immunostaining, but only at early stages of development.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.77130 |
| Date | January 1982 |
| Creators | Laurie, Gordon W. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Anatomy) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000137944, proquestno: AAINK57995, Theses scanned by UMI/ProQuest. |
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