The arachidonic acid released post-mortem from the complex lipids of rat forebrain labelled with {('3)H(,8)}-arachidonic acid had a specific activity close to that of the total phospholipids and much lower than that of the neutral lipids. The arachidonic acid is probably released from a mixture of phospholipids which excludes phosphatidylserine and phosphatidic acid. In the microsomal fraction, the specific activity of free arachidonic acid was very close to that of phosphatidylinositol. In rat cerebral cortex slices incubated in the presence of {('2)H(,8)}-arachidonic acid, prostaglandin F(,2(alpha)) reached maxiumum labelling before any of the lipids examined except for a pool of rapidly labelled free arachidonic acid located at the damaged surfaces of the tissue. In homogenates the deuterium to protium ratio of the total free arachidonic acid was similar to that of prostaglandin F(,2(alpha)). However, the probable occurrence of an isotope enrichment effect suggests that the total free arachidonic acid fraction is not identical with the precursor pool used for prostaglandin F(,2(alpha)) synthesis. In vivo, the amount of free arachidonic acid and prostaglandin F(,2(alpha)) in rat forebrain was increased following convulsions induced by carbachol and metrazol. The level of thromboxane B(,2) was not affected. Post-mortem levels of unesterified fatty acids were decreased in forebrains of phenytoin treated rats.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.68502 |
| Date | January 1979 |
| Creators | Marion, Jean. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Biochemistry) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000090045, proquestno: AAINK50502, Theses scanned by UMI/ProQuest. |
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