A colloid-free, reduced pressure in vitro normothermic perfusion system was developed to assess rabbit kidney function following cryobiological manipulation. Rabbit kidneys retained significant levels of function with stable or increasing GFR, sodium reabsorption of 40 to 60%, and glucose reabsorption of greater than 80% of the filtered load. / Including dextran in the perfusate as a source of colloid osmotic pressure was deleterious to kidney function. The kidney demonstrated sensitivity to exogenous energy substrate; supplementation of lactate and glucose resulted in better function over butyrate with glucose or glucose alone. The system proved sensitive to ischemia and to stimulation with an antidiuretic preparation. This system was utilized to examine the effects of treatment of rabbit kidneys with dimethylsulfoxide(Me(,2)SO). A recirculating constant pressure hypothermic (10(DEGREES)C) perfusion apparatus was assembled and kidneys were perfused with potassium-rich, sodium-poor solution with elevated glucose and mannitol. The pharmacokinetics of 3 M Me(,2)SO equilibration was studied through estimation of the inulin and Me(,2)SO spaces using radiochemical markers. Medullary tissue equilibration was achieved prior to cortical equilibration, which was complete after 35 minutes of exposure to 3 M Me(,2)SO. Me(,2)SO perfusion resulted in increased fluid retention by the kidney. / Function of rabbit kidneys was assessed at normothermia following Me(,2)SO introduction at two different rates, 35 minute equilibration at 3 M Me(,2)SO, and removal by an osmotic counterbalance system commencing with either 800 or 600 mOs/kg washout solution made hypertonic with mannitol. / Kidneys exhibited excellent in vitro function following perfusion with Me(,2)SO. Washout with solutions of hyperosmolarity commencing at 600 mOs/kg with gradual and stepwise reduction to 400 mOs/kg allowed immediate removal of Me(,2)SO without damage; initiation of washout at 800 mOs/kg resulted in reduced function at 37(DEGREES)C.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.68586 |
| Date | January 1981 |
| Creators | Segal, Neil B. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Animal Science) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000137424, proquestno: AAINK52120, Theses scanned by UMI/ProQuest. |
Page generated in 0.0018 seconds