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The Dox-pDC - A murine conditionally immortalized plasmacytoid dendritic cell line with native immune profile

Plasmacytoid dendritic cells (pDC) constitute a very rare blood cell population and play a significant role in immune response and immune-mediated disorders. Investigations on primary pDCs are hindered not only due to their rarity but also because they represent a heterogeneous cell population which is difficult to culture ex vivo. We generated a conditionally immortalized pDC line (Dox-pDC) from mice with Doxycycline-inducible SV40 Large T Antigen with a comparable immune profile to primary pDCs. The Dox-pDC secrete pro- and anti-inflammatory cytokines upon Toll-like receptor 9 stimulation and upregulate their MHCI, MHCII and costimulatory molecules. Further, the Dox-pDC activate and polarize naïve T cells in vivo and in vitro in response to the model antigen Ovalbumin. Due to their long-term culture stability and their robust proliferation Dox-pDC represent a reliable alternative to primary mouse pDC.

Identiferoai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:30938
Date11 June 2018
CreatorsThieme, Sebastian, Holzbaur, Alexander, Wiedemuth, Ralf, Binner, Aline, Navratiel, Katrin, Anastassiadis, Konstantinos, Brenner, Sebastian, Richter, Cornelia
PublisherPublic Library of Science
Source SetsHochschulschriftenserver (HSSS) der SLUB Dresden
LanguageEnglish
Detected LanguageEnglish
Typedoc-type:article, info:eu-repo/semantics/article, doc-type:Text
Rightsinfo:eu-repo/semantics/openAccess
Relation10.1371/journal.pone.0192437

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