La fitoterapia busca alternativas de solución a enfermedades bucales, con hierbas medicinales, como productos económicos y prácticos.La Copaifera es uno de los géneros de plantas medicinales más importantes en la región amazónica y Brasil y puede tener efecto antimicrobiano sobre las principales bacterias causantes de la enfermedad gingival y periodontal.
Objetivo: Determinar el efecto antimicrobiano de la óleo-resina de Copaifera officinalis sobre las principales cepas bacterianas periodontopatógenas (ATCC) Porphyromona gingivalis y Fusobacterium nucleatum a diversas concentraciones. Material y método: Se empleó la técnica de dilución en agar que permite hallar la concentración inhibitoria mínima. Se emplearon cepas ATCC de Porphyromona gingivalis y Fusobacterium nucleatum y la óleo-resina de Copaifera officinalis a 10 concentraciones: 100%, 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%. 0.39%, 0.19%. Se empleó la clorhexidina al 0.12% como control positivo, al agar schaedler puro y Tween 80 como controles negativos. Se emplearon 13 placas y se duplicó el ensayo. Resultados: La concentración inhibitoria mínima para Porphyromona gingivalis corresponde a 1.56%. Asimismo, la concentración inhibitoria mínima para el Fusobacterium nucleatum corresponde a 50% de óleo-resina.La clorhexidina mostró mayor efectividad al tener efecto a una menor concentración.
Conclusiones:La óleo-resina de Copaifera officinalis presentó efecto antimicrobiano frente a la cepa ATCC de Porphyromona gingivalis y Fusobacterium nucleatum, siendo el primero más sensible a su acción y el segundo más resistente. / Fitotherapy searches alternatives to solve oral diseases, with medicinal plants, as practical and low cost products. Copaifera is one the most important gender of medicinal plants in the Amazon region and Brazil. It might have an antimicrobial activity against major perioontopathogenic bacteria that gingivitis and periodontitis.
Objective: Determine the antimicrobial activity of the oleoresin of Copaifera officinalis against major periodontopathogenic bacteria (ATCC) Porphyromona gingivalis and Fusobacterium nucleatum at different concentrations.
Materials and methods: The agar dilution technique was used to determine the minimal inhibitory concentration. Porphyromona gingivalis and Fusobacterium nucleatum ATCC were used. The oleoresin at 10 concentrations::100%, 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%. 0.39%, 0.19%. Clorhexidine 0.12% was used as positive control, pure Schaedler agar and Tween 80 as negative controls. 13 plates were used and the experiment was duplicated.
Results: the minimal inhibitory concentration for Porphyromona gingivalis was 1.56%. The minimal inhibitory concentration for Fusobacterium nucleatum was 50% of the concentration of the oleoresin. Clorhexidine was more effective at a lower concentration.
Conclusions: The oleoresin of Copaifera officinalis showed antimicrobial effect against Porphyromona gingivalis and Fusobacterium nucleatum, the first one was more sensitive and the second one was more resistant to the action of the oleoresin. / Tesis
| Identifer | oai:union.ndltd.org:Cybertesis/oai:cybertesis.unmsm.edu.pe:cybertesis/3460 |
| Date | January 2013 |
| Creators | García Arroyo, Raúl Leonel |
| Contributors | Álvarez Paucar, María Angélica |
| Publisher | Universidad Nacional Mayor de San Marcos |
| Source Sets | Universidad Nacional Mayor de San Marcos - SISBIB PERU |
| Language | Spanish |
| Detected Language | Spanish |
| Type | info:eu-repo/semantics/bachelorThesis |
| Format | application/pdf |
| Source | Universidad Nacional Mayor de San Marcos, Repositorio de Tesis - UNMSM |
| Rights | info:eu-repo/semantics/openAccess |
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