The development of oocytes of differing quality retrieved using transvaginal follicular aspiration (TVFA) and following DNA injection was examined. Eight cows were subjected to twice weekly TVF A for 16 wk. Oocytes retrieved were graded and placed in an in-vitro maturation, fertilization and co-culture (IVMIIVFIIVC) program. Two thirds of oocytes were injected with DNA. Good quality oocytes from slaughtered cows (SH) were obtained once monthly and processed the same way. Good quality TVF A oocytes had a higher mean development score than poor quality oocytes, but not different from that of good quality SH oocytes. Good quality TVF A oocytes produced more viable embryos (31.7% blastocysts) than poor quality oocytes or SH oocytes (12.8% and 20.4% blastocysts, respectively). Embryo development following injection of DNA was the same for oocytes for each source-quality group (TVF A-good, 8.4; TVF A-poor, 5.5; SH-good, 6.3 % blastocysts). Development of good quality TVFA oocytes increased during the last 9 wk of the 16 wk collection period. Poor oocyte development increased slightly to 9 wk and then decreased. Development of TVF A oocytes injected with DNA did not vary during the experiment. However, development of controls increased from a mean score of2.50 at wk 1 to 4.17 at wk 16. Oocytes from TVFA produced more PCR positive blastocysts (95.0%) than SH oocytes (61.5%). More calves were born from the transfer of embryos injected with DNA from TVF A oocytes (3/5) than from SH oocytes (116), although not statistically significant. One calf was PCR positive in bone-marrow, but was negative in other tissues. The use of oocytes obtained by TVF A may improve the efficiency of producing transgenic cattle. / Master of Science
| Identifer | oai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/44483 |
| Date | 29 August 2008 |
| Creators | Garst, Amy S. |
| Contributors | Dairy Science, Gwazdauskas, Francis C., Akers, Robert Michael, Pearson, Ronald E., Vinson, William E. |
| Publisher | Virginia Tech |
| Source Sets | Virginia Tech Theses and Dissertation |
| Language | English |
| Detected Language | English |
| Type | Thesis, Text |
| Format | vii, 70 leaves, BTD, application/pdf, application/pdf |
| Rights | In Copyright, http://rightsstatements.org/vocab/InC/1.0/ |
| Relation | OCLC# 36120241, LD5655.V855_1996.G377.pdf |
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