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Evaluation of Diet, Water, and Culture Size for Ceriodaphnia Dubia Laboratory Culturing

Six reagent waters, eleven diets, and two culture sizes were evaluated for culturing C. dubia. Different filtration techniques were used to prepare the reagent waters. The eleven diets were comprised of two algae augmented with eight supplements. Reproduction and growth were assessed to discern differences among C. dubia raised in mass cultures and cultured in individual cups, during which, bacterial population densities, lipid, protein, and carbohydrate concentrations of the diets were measured. Results showed that a glass-distilled, carbon filtered, deionized reagent water and a Selenastrum capricornutum- Cerophyl® diet were optimum for culturing. Mass culturing supported the highest reproduction and growth, while no correlation was found between nutritional measurements and production.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc501234
Date12 1900
CreatorsAllen, Jerry D. (Jerry Dee)
ContributorsDickson, Kenneth L., Waller, William, Kennedy, James H.
PublisherUniversity of North Texas
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatix, 108 leaves: ill., Text
RightsPublic, Allen, Jerry D. (Jerry Dee), Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved.

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