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Previous issue date: 2010-12-02 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The microorganisms play very important roles in maintaining ecosystems, which explains the enormous interest in understanding the relationship between these organisms as well as between them and the environment. It is estimated that the total number of prokaryotic cells on Earth is between 4 and 6 x 1030, constituting an enormous biological and genetic pool to be explored. Although currently only 1% of all this wealth can be cultivated by standard laboratory techniques, metagenomic tools allow access to the genomic potential of environmental samples in a independent culture manner, and in combination with third generation sequencing technologies, the samples coverage become even greater. Soils, in particular, are the major reservoirs of this diversity, and many important environments around us, as the Brazilian biomes Caatinga and Atlantic Forest, are poorly studied. Thus, the genetic material from environmental soil samples of Caatinga and Atlantic Forest biomes were extracted by direct techniques, pyrosequenced, and the sequences generated were analyzed by bioinformatics programs (MEGAN MG-RAST and WEBCarma). Taxonomic comparative profiles of the samples showed that the phyla Proteobacteria, Actinobacteria, Acidobacteria and Planctomycetes were the most representative. In addition, fungi of the phylum Ascomycota were identified predominantly in the soil sample from the Atlantic Forest. Metabolic profiles showed that despite the existence of environmental differences, sequences from both samples were similarly placed in the various functional subsystems, indicating no specific habitat functions. This work, a pioneer in taxonomic and metabolic comparative analysis of soil samples from Brazilian biomes, contributes to the knowledge of these complex environmental systems, so far little explored / Os microorganismos desempenham importantes fun??es na manuten??o dos ecossistemas, o que explica o enorme interesse em compreender as rela??es existentes entre estes organismos, bem como entre eles e o meio. Estima-se que o n?mero total de c?lulas procari?ticas na Terra seja entre 4 e 6 x 1030, constituindo um enorme pool biol?gico e gen?tico a ser explorado. Apesar de atualmente apenas 1% de toda essa riqueza poder ser cultivada por t?cnicas laboratoriais padr?o, ferramentas metagen?micas permitem o acesso ao potencial gen?mico de amostras ambientais de forma independente de cultivo, e em associa??o com tecnologias de sequenciamento da terceira gera??o, a cobertura amostral se torna ainda maior. Solos, em particular, s?o os maiores reservat?rios dessa diversidade, e muitos ambientes importantes ao nosso redor, como os biomas brasileiros Caatinga e Mata Atl?ntica, s?o pouco estudados. Sendo assim, o material gen?tico ambiental de amostras de solo dos biomas Caatinga e Mata Atl?ntica foi extra?do atrav?s de t?cnicas diretas, pirosequenciado, e as seq??ncias geradas foram analisadas atrav?s de programas de bioinform?tica (MEGAN, MG-RAST e WEBCarma). Perfis taxon?micos comparativos das amostras mostraram que os filos Proteobacteria, Actinobacteria, Acidobacteria e Planctomycetes foram os mais representativos. Em adi??o, fungos do filo Ascomycota foram identificados predominantemente na amostra de solo de Mata Atl?ntica. Perfis metab?licos mostraram que, apesar da exist?ncia de diferen?as ambientais, sequ?ncias de ambas as amostras foram inseridas similarmente nos diversos subsistemas funcionais, n?o indicando fun??es habitat espec?ficas. Este trabalho, pioneiro em an?lises taxon?micas e metab?licas comparativas de amostras de solo de biomas brasileiros, contribui para o conhecimento destes sistemas ambientais complexos, at? ent?o pouco explorados
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/12572 |
| Date | 02 December 2010 |
| Creators | Pacchioni, Ralfo Goes |
| Contributors | CPF:00297997750, http://lattes.cnpq.br/1083882171718362, Ara?jo, Magn?lia Fernandes Flor?ncio de, CPF:51274957400, http://lattes.cnpq.br/4511989641459455, Melo, V?nia Maria Maciel, CPF:11269545353, http://lattes.cnpq.br/1572504650930605, Lima, Lucymara Fassarela Agnez |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Bioqu?mica, UFRN, BR, Bioqu?mica; Biologia Molecular |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/embargoedAccess |
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