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Effects of Porcine Relaxin Hormone on Motility Characteristics of Boar Spermatozoa during Storage

First, a preliminary study was conducted looking for the optimum sperm concentration to be used for analysis with the Computer-Assisted Sperm Analysis (CASA). Results showed that 75x106 sperm cells/mL is the optimum one. Then, the actions of relaxin on sperm motility were evaluated by determining the effect of relaxin on full motility characteristics of spermatozoa during storage, using CASA; then identifying the relaxin receptors on spermatozoa, and finally establishing actions of relaxin in intraspermatic cAMP content. Motile spermatozoa were selected through percoll gradient and incubated for 1 hour with 4 relaxin concentrations at 37°C, during four days. Relaxin affected sperm motility (P<0.05). This action appears associated with the presence of relaxin receptors RXFP1 and RXFP2 that were found in spermatozoa. However, the cAMP levels were not affected by relaxin (P<0.05). This study indicates a beneficial action of relaxin on sperm motility; however, its mechanism of action requires further research.

Identiferoai:union.ndltd.org:MSSTATE/oai:scholarsjunction.msstate.edu:td-2777
Date30 April 2011
CreatorsRodríguez Muñoz, Juan Camilo
PublisherScholars Junction
Source SetsMississippi State University
Detected LanguageEnglish
Typetext
Formatapplication/pdf
SourceTheses and Dissertations

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