DNA molecules stretching and deformation in hydrodynamic flow field has been investigated for many years with various experimental and theoretical methods. This research was performed experimentally in hybridized microchannels made of poly(dimethylsiloxane)(PDMS) and glass. We will directly measure the hydrodynamic stretching DNA molecules in elongation flow by CLSM (Confocal Laser Scanning Microscope). The deformation of YOYO-labeled lambda phage DNA molecules in flows was visualized with CLSM. The relation between DNA contour length and relaxation time in different models of stretching flows was also be presented. Furthermore, the distribution of stretching DNA molecules in microchannels was derived to analyze and compared with the DNA migration velocity and stress relaxation modulus.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0721106-170554 |
| Date | 21 July 2006 |
| Creators | Liu, Chang-hao |
| Contributors | Chin-Chia Su, Shou-Shing Hsieh, Shou-Shing Hsieh, Ching-Jenq Ho |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0721106-170554 |
| Rights | unrestricted, Copyright information available at source archive |
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