Weanalyze the mechanical properties of three epithelial/mesenchymal cell lines (MCF-10A, MDAMB-
231, MDA-MB-436) that exhibit a shift in E-, N- and P-cadherin levels characteristic of an
epithelial−mesenchymal transition associated with processes such as metastasis, to quantify the role of
cell cohesion in cell sorting and compartmentalization. Wedevelop a unique set of methods to
measure cell–cell adhesiveness, cell stiffness and cell shapes, and compare the results to predictions
from cell sorting in mixtures of cell populations.Wefind that the final sorted state is extremely robust
among all three cell lines independent of epithelial or mesenchymal state, suggesting that cell sorting
may play an important role in organization and boundary formation in tumours.Wefind that surface
densities of adhesive molecules do not correlate with measured cell–cell adhesion, but do correlate
with cell shapes, cell stiffness and the rate at which cells sort, in accordance with an extended version of
the differential adhesion hypothesis (DAH). Surprisingly, theDAHdoes not correctly predict the final
sorted state. This suggests that these tissues are not behaving as immiscible fluids, and that dynamical
effects such as directional motility, friction and jamming may play an important role in tissue
compartmentalization across the epithelial−mesenchymal transition.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:80389 |
| Date | 12 August 2022 |
| Creators | Pawlizak, Steve, Fritsch, Anatol W., Grosser, Steffen, Ahrens, Dave, Thalheim, Tobias, Riedel, Stefanie, Kießling, Tobias R., Oswald, Linda, Zink, Mareike, Manning, M. Lisa, Käs, Josef A. |
| Publisher | IOP Publishing |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 083049 |
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