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Previous issue date: 2015-03-16 / Os v?rus dengue pertence ? fam?lia Flaviviridae e ao g?nero Flavivirus, sendo composto por 4
sorotipos antigenicamente distintos, s?o considerados os arbov?rus mais importantes no
mundo por causar altas taxas de morbidade e mortalidade em regi?es tropicais e subtropicais
do planeta, colocando em risco at? 3,6 bilh?es de pessoas em mais de 100 pa?ses. Por ser uma
doen?a com amplo espectro cl?nico e por n?o possuir vacina ou tratamento eficaz, o estudo de
poss?veis antivirais que visam diminuir a viremia do paciente ? de suma import?ncia, j? que
este ? um dos fatores que pode levar a febre hemorr?gica da dengue e a s?ndrome do choque
da dengue que s?o as formas grave da doen?a. No presente estudo foi avaliado o potencial
antiviral do extrato da folhada planta Caesalpinia echinata contra o v?rus dengue-2 (DENV-2)
em cultura de c?lulas C6/36 e Vero e a a??o antiviral da Rifampicina em c?lulas Vero. A
escolha da Caesalpinia echinata se deve ao fato de que j? foi observada sua a??o antiinflamat?ria
e antimal?rica, al?m de n?o ter sido encontrado nenhum trabalho que tenha
avaliado seu potencial de a??o frente a v?rus. A Rifampicina foi escolhida por demonstrado
a??o antiviral, principalmente contra os poxvirus, por?m poucos s?os os relatos da utiliza??o
deste f?rmaco contra v?rus de RNA. O resultado foi obtido atrav?s da quantifica??o da carga
viral pela t?cnica da qRT-PCR em Tempo Real. As c?lulas infectadas por DENV-2 foram
submetidas ao tratamento pelo per?odo de 7 dias em diferentes concentra??es do extrato da
planta Caesalpinia echinataque variou entre 0,68 a 0,0068mg/mL. N?o foi poss?vel observar
neste estudo, evid?ncias de inibi??o significativa da replica??o do v?rus DENV-2 em ambas as
culturas celulares. ARifampicina foi utilizada em diferentes condi??es de tratamento, no qual
foi avaliado ao longo de 72 horas a carga viral produzida nas c?lulas Vero.Nas condi??es de
tratamento p?s-infec??o e no ensaio virucida o f?rmaco apresentou atividade antiviral,
reduzindo a taxa de replica??o em 100 vezes em rela??o ao controle. De acordo com os
nossos resultados conclui-se que a Rifampicina mostrou-se eficaz no combate a infec??o do
DENV-2 em cultura de c?lulas Vero. / The dengue virus belongs to the Flaviviridae family and the Flavivirus genus, consisting of
four serotypes antigenically distinct, are considered the most important arbovirus in the world
to cause high rates of morbidity and mortality in tropical and subtropical regions of the world,
threatening to 3, 6 billion people in over 100 countries. Because it is a disease with a wide
clinical spectrum and has no vaccine or effective treatment, the study of possible antiviral
drugs aimed at reducing viremia of patients is of paramount importance, since this is one of
the factors which can lead to dengue hemorrhagic fever and dengue shock syndrome that are
severe forms of the disease. In the present study we evaluated the antiviral potential of the leaf
extract of the plant Caesalpinia echinata against dengue-2 virus (DENV-2) in cultured C6/36
and Vero cells and the antiviral action of Rifampicin on Vero cells. The choice of Caesalpinia
echinata is due to the fact that has been observed its action anti-inflammatory and antimalarial
, and not have been found no study evaluated its antiviral effect. Rifampin was
chosen was chosen for demonstrated antiviral action, especially against poxviruses, but few
sane reports usage of this drug against RNA viruses. The result was obtained by quantifying
the viral load bythe technique of Real-time qRT-PCR. Cells infected with DENV-2 were
subjected to treatment for 7 days in different concentrations of plant extract Caesalpinia
echinata (0.68 - 0,0068mg / ml). As a result, we could not observe a inhibition significant of
virus replication in both cell cultures. The Rifampicin was used for different treatment
condition,which were evaluated over 72 hours the amount of viral load produced in Vero
cells, In the conditions treatment and the test virucidal theantiviral activity, which is capable
of reducing the rate of 100X replication as compared to control. According to our results we
can conclude that Rifampicin was effective in action against to infection DENV-2 in Vero cell
culture.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/20321 |
| Date | 16 March 2015 |
| Creators | Almeida J?nior, Renato Ferreira de |
| Contributors | 58068660259, http://lattes.cnpq.br/9145835635441776, Silva, Francisco F?bio Marques da, 81394209487, http://lattes.cnpq.br/5313960881849449, Andrade, V?nia Sousa, 56108150610, http://lattes.cnpq.br/9327124853897215, Ara?jo, Jos?lio Maria Galv?o de, Farias, Kleber Juvenal Silva |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM CI?NCIAS BIOL?GICAS, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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