The overexpression of the enzymes involved in the degradation of procollagen lysine is
correlated with various tumor entities. Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (PLOD3)
expression was found to be correlated to the progression and migration of cancer cells in gastric,
lung and prostate cancer. Here, we analyzed the gene expression, protein expression, and the clinical
parameters of survival across 33 cancers based on the Clinical Proteomic Tumor Analysis Consortium
(CPTAC), function annotation of the mammalian genome 5 (FANTOM5), Gene Expression Omnibus
(GEO), Genotype-Tissue Expression (GTEx), Human Protein Atlas (HPA) and The Cancer Genome
Atlas (TCGA) databases. Genetic alteration, immune infiltration and relevant cellular pathways were
analyzed in detail. PLOD3 expression negatively correlated with survival periods and the infiltration
level of CD8+ T cells, but positively correlated to the infiltration of cancer associated fibroblasts in
diverse cancers. Immunohistochemistry in colon carcinomas, glioblastomas, and soft tissue sarcomas
further confirm PLOD 3 expression in human cancer tissue. Moreover, amplification and mutation
accounted for the largest proportion in esophageal adenocarcinoma and uterine corpus endometrial
carcinoma, respectively; the copy number alteration of PLOD3 appeared in all cancers from TCGA;
and molecular mechanisms further proved the effect of PLOD3 on tumorigenesis. In particular,
PLOD3 expression appears to have a tumor immunological effect, and is related to multiple immune
cells. Furthermore, it is also associated with tumor mutation burden and microsatellite instability in
various tumors. PLOD3 acts as an inducer of various cancers, and it could be a potential biomarker
for prognosis and targeted treatment.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:89199 |
| Date | 23 January 2024 |
| Creators | Gong, Siming, Duan, Yingjuan, Wu, Changwu, Osterhoff, Georg, Schopow, Nikolas, Kallendrusch, Sonja |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 9903 |
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