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Previous issue date: 2016-02-19 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Os genes de reparo do DNA s?o essenciais para manuten??o da integridade do genoma, evitando graves doen?as como o c?ncer. O papel de v?rias prote?nas codificadas por esses genes vem sendo associado tanto ao risco do desenvolvimento, como na evolu??o de variados c?nceres humanos, dentre eles, o carcinoma epidermoide oral. O objetivo deste trabalho foi analisar a imunoexpress?o das prote?nas de reparo do DNA, XRCC1, THIIF e XPF em carcinoma epidermoide de l?ngua oral e investigar associa??o com par?metros cl?nicos, histopatol?gicos e de desfecho. Tamanho do tumor, comprometimento linfonodal, est?gio do tumor, profundidade de invas?o >4mm e o sistema de grada??o de Almangush, mostraram-se como fatores progn?sticos. Evidenciou-se de uma maneira geral, alta express?o imuno-histoqu?mica das prote?nas de reparo nas c?lulas parenquimatosas; no entanto, apenas verificou-se associa??o significativa da elevada express?o de XRCC1 com melhor estadiamento cl?nico. Os resultados deste experimento sugerem que as prote?nas XRCC1, TFIIH e XPF participam do processo de tumorig?nese, entretanto a imunoexpress?o das mesmas n?o pode ser utilizada como indicador progn?stico para o carcinoma epidermoide de l?ngua oral. / DNA repair systems, genes and proteins are essential for genome integrity maintenance, avoiding serious diseases such as cancer. Deregulation in the expression of those proteins has been associated with both the risk of development and evolution of various human cancers, including oral squamous cell carcinoma. The purpose of this study was to analyze the immunoreactivity of the DNA repair proteins XRCC1, THIIF and XPF in oral tongue squamous cell carcinoma (OTSCC) and to investigate its association with clinical and histopathological parameters, outcome and 5-year survival rate. Seventy-four cases of OTSCC were analyzed semi-quantitatively through immunohistochemistry. We observed that DNA repair proteins were highly expressed in parenchymal cells; however, we only observed a significant association between XRCC1 high expression and better clinical staging (p=0,02). Cox regression showed that tumor size (p<0,01), lymph node involvement (p=0,04), tumor stage (p=0,02) and depth of invasion> 4mm (p=0,05) were prognostic factors. The results of this experiment suggest that XRCC1, TFIIH and XPF participate in the tumorigenic process, however, their immunoexpression may not be used as an independent prognostic indicator for OTSCC.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/21014 |
| Date | 19 February 2016 |
| Creators | S?, Melka Co?lho |
| Contributors | 28444361453, http://lattes.cnpq.br/9512014003639405, Ribeiro, Betania Fachetti, 07027833743, http://lattes.cnpq.br/4345846278905672, Santos, Bruna Rafaela Martins dos, 01163265497, http://lattes.cnpq.br/2611005967205110, Gurgel, Bruno Cesar de Vasconcelos, 02569549410, http://lattes.cnpq.br/4649601602612601, Pinto, Le?o Pereira, 01608789420, http://lattes.cnpq.br/7040457616034306, Freitas, Roseana de Almeida |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM PATOLOGIA ORAL, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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