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Previous issue date: 2010-02-23 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior-CAPES / Brazil was adverted by the World Health Organization (WHO) as a sub-clinical area of
serious vitamin A deficiency. Thus, the Brazilian Agricultural Research Corporation
(EMBRAPA) is selecting and improving varieties of sweet potatoes with higher levels
of Beta-carotene, pro-vitamin A. The carotenoids provitamin A amount of in foods does
not necessarily correspond the amount that is absorbed and metabolized by the body.
For a better determination of these values and knowledge of the mechanisms of its
transport and absorption, it is necessary to understand the factors that lead to the food
matrix release, until the absorption and the influence on the promotion and maintenance
in human health. In order to perform preliminary studies of its absorption, this study
aims to determine the efficiency of micellization of Beta -carotene in orange sweet potato
(Ipomoea batatas, Lam), by applying a in vitro digestion as a tool for determining the
bioaccessibility, which is the first step for bioavailability determination. This approach
aims to simulate the oral, gastric and intestinal stages of human. The digestion was
performed with 10 g of fresh samples, homozeneided with 5% (w/w) of canola oil. The
extraction was performed with acetone and petroleum ether and the micellar fraction
with petroleum ether, NaCl 10% (w / v) and NaSO4 2% (w/v) and involves the use of
enzymes as -amylase , pepsin, bile, pancreatin, lipase and mucin, and inorganic
compounds such as KCl, KSCN, NaH2PO4, Na3PO4, NaOH, NaCl, CaCl2, HCl,
NaHCO3. The physiological variations are reproduced by the heating bath shaker with
orbital gyrus (37?C) and centrifugation (5000g, 45 min). Quantification and
determination of the profile of carotenoids were performed by high performance liquid
chromatography (HPLC) with YCM ? C30 Carotenoid S-3 4.6 x 250mm column.
Quantification of total carotenoids was performed by UV-VIs. The whole procedure
was performed under controlled temperature (25 ? C) and light. The Beta -carotene was
present mainly with levels of 86%, 73% and 82% for access 1, 2 and 3 and after
digestion, the profile of Beta -carotene has set levels of 96%, 89% and 100%, respectively.
The efficiency of micellization was 23.8%, 28% and 25% for 1.2 and 3 hits, indicating
Beta -carotene transfer of the food matrix to micelles, corresponding to bioaccessibility of
the compound. This methodology proved to be faster and cheaper, since the in vivo
studies are costly, complex and require more time. / O Brasil foi classificado pela Organiza??o Mundial da Sa?de (OMS) como ?rea de
car?ncia sub-cl?nica grave de vitamina A. Assim, a Empresa Brasileira de Pesquisa
Agropecu?ria (EMBRAPA) v?m selecionando e melhorando variedades de batata-doce
com teores maiores de Beta-caroteno, composto pr?-vitamina A. A quantidade de
caroten?ides pr?-vitamina A presentes nos alimentos n?o corresponde necessariamente
?quela quantidade absorvida e metabolizada pelo organismo. Para uma melhor
determina??o destes valores e conhecimento dos mecanismos de transporte e absor??o
deste composto, faz-se necess?rio, o entendimento dos fatores que levam ? sua libera??o
da matriz do alimento, at? a extens?o de sua absor??o, bem como a influ?ncia na
promo??o e manuten??o da sa?de humana. A fim de realizar estudos preliminares de
sua absor??o, este trabalho visa determinar a efici?ncia de miceliza??o de Beta-caroteno de
batata-doce de polpa alaranjada (Ipomoea batatas, Lam.), atrav?s da aplica??o de
digest?o in vitro, como ferramenta de determina??o da bioacessibilidade, etapa
preliminar para a determina??o da biodisponibilidade. Esta metodologia visa simular as
etapas de digest?o oral, g?strica e intestinal humana. Foram pesados 10g de amostra in
natura e adicionados 5% (p/p) de ?leo de canola. A extra??o do alimento foi realizada
com acetona e ?ter de petr?leo e a da fra??o micelar, com ?ter de petr?leo, NaCl
10%(p/v) e NaSO4 2%(p/v) . Enzimas como: a-amilase, pepsina, bile, pancreatina,
lipase e mucina, bem como compostos inorg?nicos, tais como KCl, KSCN, NaH2PO4,
Na3PO4, NaOH, NaCl, CaCl2, HCl, NaHCO3. As varia??es fisiol?gicas foram
reproduzidas pelo banho de aquecimento com giro orbital (37?C) e centrifuga??o
(5000g;45 min). A quantifica??o e determina??o do perfil de caroten?ides foi realizada
por cromatografia l?quida de alta efici?ncia (CLAE), com coluna YCM? Carotenoid C30
S-3 de 4,6 x 250mm. A quantifica??o de caroten?ides totais foi realizada por
espectrofotometria UV-VIs. Todo o procedimento foi executado sob temperatura (25?C)
e luz controlada. O Beta-caroteno estava presente majoritariamente, com teores de 86%,
73% e 82%, para as variedades 1, 2 e 3 e ap?s a digest?o, o perfil do Beta-caroteno passou
a configurar teores de 96%, 89% e 100%, respectivamente. A efici?ncia de miceliza??o
foi de 23,8%, 28% e 28,9% para as variedades 1,2 e 3, indicando a transfer?ncia do -
caroteno da matriz do alimento para as micelas, correspondendo a bioacessibilidade
deste composto. Esta metodologia demonstrou-se mais r?pida e mais barata, quando
comparada aos estudos in vivo, que s?o mais onerosos, complexos e demandam mais
tempo.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/1186 |
| Date | 23 February 2010 |
| Creators | Giori, Fernanda Peixoto |
| Contributors | Godoy, Ronoel Luiz de Oliveira, Moura, Mirian Ribeiro Leite, Ascheri, Jos? Luis Ram?rez, Borguini, Renata Galhardo |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos, UFRRJ, Brasil, Instituto de Tecnologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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