The thesis begins with an introduction to signal transduction
and an analysis of current understanding of plant signal
transduction. There are similarities between plants and animals,
but also key differences, including lack of protein kinase C and of a
cAMP signaling pathway in plants, and presence in plants of
calcium dependent protein kinase (CDPK), which has a kinase
catalytic domain contiguous with a C-terminal calmodulin-like
domain. The next section examines protein kinase activity in the
plasma membrane (PM) of zucchini hypocotyls. Zucchini PM
contains four or more polypeptides with calcium-requiring protein
kinase activity. The enzymes appear to be tightly associated with
the PM, and at least three are recognized by monoclonal antibody
to soybean soluble CDPK. Total proteins from several different
organs of zucchini seedlings contain kinases with molecular
weights similar to the hypocotyl PM enzymes. In the third section
details of partial purification of the solubilized PM kinases are
presented. Kinases which do not crossreact with anti-CDPK
monoclonal antibody were resolved by anion exchange from ones
which do crossreact. Peptide mapping was used to test the
relationship between the kinases. Results of peptide mapping
suggest that at least three types of protein kinase are present in
zucchini PM, two of which are immunologically similar to CDPK and
one of which is not. The last section concerns the potential for
testing interactions between PM protein kinases and plasma
membrane auxin binding proteins (ABP's) by use of photoaffinity
labeling of ABP's. Causes of variable photoaffinity labeling by an
azido-IAA are considered. Labeling of both the tomato mutant
diageotropica and the parent VFN membranes was inexplicably
inconsistent. / Graduation date: 1994
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/35700 |
| Date | 17 August 1993 |
| Creators | Verhey, Steven D. |
| Contributors | Lomax, Terri L. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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