Temperature has a reliable and nearly instantaneous influence onmechanical responses of cells.As recently
published, MCF-10Anormal epithelial breast cells follow the time–temperature superposition (TTS)
principle. Here,wemeasured thermorheological behaviour of eightcommoncell types within
physiologically relevant temperatures and appliedTTS to creep compliance curves.Our results showed that
superposition is not universal and was seen in four of the eight investigated cell types. For the other cell
types, transitions of thermorheological responses were observed at 36 °C.Activation energies (EA)were
calculated for all cell types and ranged between 50 and 150 kJmol−1.The scaling factors of the superposition
of creep curves were used to group the cell lines into three categories. They were dependent on relaxation
processes aswell as structural composition of the cells in response tomechanical load and temperature
increase.This study supports the view that temperature is a vital parameter for comparing cell rheological
data and should be precisely controlledwhen designing experiments.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:80387 |
| Date | 12 August 2022 |
| Creators | Schmidt, Sebastian, Kießling, Tobias R., Warmt, Enrico, Fritsch, Anatol W., Stange, R., Käs, Josef A. |
| Publisher | IOP Publishing |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 073010 |
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