Although nano-sized metal colloids are used in industrial and medicinal
applications, little is known about the potential liver toxicity of these materials
after occupational or intentional exposures. To begin to resolve some
outstanding hepatotoxicity concerns, the inflammatory response of hepatocytes
after exposure to metal colloids was assessed. Four ~30-nm-sized metal
colloids, including silver (nano-Ag), copper (nano-Cu) and nickel (nano-Ni) were
examined in an effort to understand the induced cytokine expression in a murine
liver cell line (AML12). Here we also utilized another system, co-cultures of
hepatocytes, Kupffer’s cells, and lymphocytes isolated from C57BL6 mice. Cells
were exposed to the materials over dose-response (0.1mg/L to 1000mg/L) and
time-dependent (4 h, 48 h, and 1-week) studies. Cytotoxicity was measured via
metabolism of resazurin and validated via MTT assay and cell counts.
Inflammatory response was determined by cytokine profiles (TNF-a and IL-6), as well as by mRNA and protein expression of heat shock protein (Hsp70). Results
from cells exposed to nano-Ag to doses of up to 100mg/L exhibited no
significant changes in cytotoxicity, IL-6, or TNF-a production, or Hsp70
expression. Both nano-Cu and nano-Ni exposed cells exhibited decreased
metabolism, increased Hsp70 induction, and increased inflammatory responses
(IL-6 and TNF-a). Dynamic light scattering and electron microscopy were used
to characterize particle size and surface charge. All three metal colloidal
systems demonstrated different particle size distributions, agglomerated sizes,
and surface zeta potentials. Furthermore, each metal colloid system elicited
different inflammatory biomarker responses and stress protein expression.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/ETD-TAMU-2010-08-8414 |
| Date | 2010 August 1900 |
| Creators | Banerjee, Nivedita |
| Contributors | Sayes, Christie M. |
| Source Sets | Texas A and M University |
| Language | en_US |
| Detected Language | English |
| Type | thesis, text |
| Format | application/pdf |
Page generated in 0.0018 seconds