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Previous issue date: 2013-03-04 / The use of medicinal plants to cure and treat various diseases is a common practice in the world and in Brazil. In several regions of the Brazil?s Northeast, the cactus Cereus jamacaru, known as mandacaru, is used popularly as a treatment to many diseases, including those related to heart respiratory diseases, gastric ulcers, scurvy, and kidney diseases. However, there is a scarcity in the scientific literature that proves scientifically the popular application of this cactus. Like other plants, Cereus jamacaru synthesizes several potentially bioactive molecules, like as polysaccharides. In this work, three polysaccharides-rich aqueous extracts, MCA80, MPM and MCP60, were obtained from this plant and analyzed chemically, as well as their cytotoxic and antioxidant potential. The data showed that all extracts consist mainly of polysaccharides (89.42 to 95.76%), but also protein (> 2%) and phenolic (3 to 8.87%) contaminants were detected. All extracts are rich in galactose, glucose and mannose. In addition, glucuronic acid was found in MCA80 and MCP60. The extracts showed total antioxidant capacity ranged from 55.21 to 68.13 of ascorbic acid equivalents (AAE). Besides, they exhibited reducer power and cupric chelation in a dose-dependent manner. None of the extracts inhibited the MTT reduction in the presence of prostate tumor cells (PC-3). However, MCP60 was the most effective extract by preventing the reduction of MTT by about 80% in the presence of cells 786. Nuclear fragmentation tests showed that this extract induces cell death. The data indicated that mandacaru synthesizes bioactive polysaccharides with potential as antioxidant and antitumor agents. For future studies, it is intended to purify and characterize these polysaccharides and its antioxidant and antitumor mechanisms / A utiliza??o de plantas medicinais para a cura e tratamento de diversas afec??es ? uma pr?tica comum no mundo e tamb?m no Brasil. Em v?rias regi?es do Nordeste brasileiro o cacto Cereus jamacaru, mais conhecido como mandacaru, ? utilizado popularmente no tratamento de v?rias doen?as, incluindo aquelas relacionadas com problemas card?acos, respirat?rios, ?lceras g?stricas, escorbuto, e problemas renais. Contudo, h? uma escassez na literatura cient?fica que comprove cientificamente a aplica??o popular do mandacaru. Assim como outras plantas, Cereus jamacaru sintetiza v?rias mol?culas potencialmente bioativas, como polissacar?deos. Neste trabalho, tr?s extratos aquosos ricos em polissacar?deos, MCA80, MPM e MCP60, foram obtidos dessa planta e analisados quimicamente, bem como, foi avaliado os seus potencias como agentes antioxidantes e citot?xicos. Os dados mostraram que todos os extratos s?o constitu?dos principalmente de polissacar?deos (89,42 a 95,76%), por?m foram detectados contaminantes proteicos (>2 %) e fen?licos (3 a 8,87%). Todos os extratos s?o ricos em galactose, glicose e manose. Al?m disso, de MCA80 e MCP60 apresentam ?cido glucur?nico em sua composi??o. Os extratos apresentaram capacidade antioxidante total variando de 55,21 a 68,13 equivalentes de ?cido asc?rbico (EAA). Por?m n?o apresentaram atividade sequestradora de ?on super?xido. Por outro lado, eles apresentaram atividade redutora e quela??o c?prica de forma dose-dependente. Nenhum dos extratos inibiu a redu??o do MTT na presen?a das c?lulas tumorais de pr?stata (PC-3). Por outro lado, as c?lulas HEK, HeLa e 786 tiveram seu poder redutor de MTT inibido em diferentes n?veis na presen?a dos extratos. MCP60 foi o extrato mais efetivo, impedindo a redu??o do MTT em cerca de 80% na presen?a das c?lulas 786. Ensaios de fragmenta??o nuclear mostraram que esse extrato induz morte celular. Os dados indicaram que mandacaru sintetiza polissacar?deos bioativos com potencial como agentes antioxidantes e antitumorais. Em estudos futuros pretende-se purificar esses polissacar?deos e caracterizar seus mecanismos de a??o antioxidante e antitumoral
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/12618 |
| Date | 04 March 2013 |
| Creators | Morais, Daniel de Souza Cruz |
| Contributors | CPF:76111830449, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799567J8&dataRevisao=null, Costa, Leandro Silva, CPF:04647720446, http://lattes.cnpq.br/4991977240761750, Bezerra, Ranilson de Souza, CPF:74436767434, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797575J5, Rocha, Hugo Alexandre de Oliveira |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Bioqu?mica, UFRN, BR, Bioqu?mica; Biologia Molecular |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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