Myc is a powerful oncogene frequently deregulated in cancer yet deregulated Myc alone does not lead to cellular transformation due to the intrinsic safety mechanism of deregulated Myc potentiating apoptosis. The mechanism by which Myc potentiates apoptosis remains unclear, however, because the regions of Myc essential for apoptosis are also required for Myc to function as a regulator of gene transcription, it is thought that Myc’s role in apoptosis is a function of its regulation of an apoptotic genetic program. We hypothesize that under apoptotic conditions, Myc differentially binds and/or regulates a specific cohort of genes to potentiate apoptosis. The foremost approach to addressing this hypothesis is the employment of ChIP-chip coupled with expression microarray analyses. Here, using the MCF10A breast epithelial and SHEP neuroblastoma cell lines, we developed and characterized two independent human systems for subsequent ChIP-chip and expression array analyses to elucidate the genetic program of Myc-potentiated apoptosis.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/18851 |
| Date | 15 February 2010 |
| Creators | Rust, Andrew |
| Contributors | Penn, Linda Z. |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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