by Cheng Chi Wa. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1998. / Includes bibliographical references (leaves 110-120). / Abstract also in Chinese. / Title --- p.i / Abstract --- p.ii / Acknowledgement --- p.iv / Abbreviations --- p.v / Abbreviation Table for Amino Acids --- p.vi / Table of Contents --- p.vii / List of Figures --- p.xii / List of Tables --- p.xv / Chapter Chapter 1 --- Introduction --- p.1 / Chapter Chapter 2 --- Literature review --- p.2 / Chapter Part I --- Hypertrophic Scar / Chapter 2.1 --- Definition of hypertrophic scar --- p.2 / Chapter 2.2 --- Pathology --- p.2 / Chapter 2.3 --- Epidemiology findings --- p.3 / Chapter 2.3.1 --- Ethnicity --- p.3 / Chapter 2.3.2 --- Age --- p.3 / Chapter 2.3.3 --- Body location --- p.3 / Chapter 2.4 --- Mechanism of cutaneous wound healing --- p.4 / Chapter 2.4.1 --- Phase I - Haemostasis and inflammation --- p.4 / Chapter 2.4.1.1 --- Haemostasis --- p.6 / Chapter 2.4.1.2 --- Early phase of inflammation --- p.6 / Chapter 2.4.1.3 --- Late phase of inflammation --- p.7 / Chapter 2.4.2 --- Phase II - Re-epithelialization --- p.7 / Chapter 2.4.2.1 --- Migration of epidermal keratinocytes --- p.8 / Chapter 2.4.2.2 --- Migration of fibroblasts --- p.8 / Chapter 2.4.2.3 --- Angiogenesis --- p.9 / Chapter 2.4.3 --- Phase III - Tissue remodeling --- p.10 / Chapter 2.4.3.1 --- Cell maturation and apoptosis --- p.10 / Chapter 2.4.3.2 --- Exrtracellular matrix remodeling --- p.10 / Chapter 2.5 --- Alteration of wound healing - Possible pathogenic factors of hypertrophic scar --- p.11 / Chapter 2.5.1 --- Changes in Phase I-Inflammation --- p.13 / Chapter 2.5.2 --- Changes in Phase II - Re-epithelialization/ tissue formation --- p.14 / Chapter 2.5.3 --- Changes in Phase III - Tissue remodeling --- p.15 / Chapter 2.6 --- The Role of fibroblasts in the formation of hypertrophic scar --- p.16 / Chapter 2.6.1 --- Functions of fibroblasts in wound healing --- p.16 / Chapter 2.6.2 --- Suggested aetiological role in the formation of hypertrophic scar fibroblasts --- p.16 / Chapter 2.6.2.1 --- Fibroproliferation disorder --- p.18 / Chapter 2.6.2.2 --- Extracellular Matrix remodeling disorder --- p.18 / Chapter a) --- CoUaqen --- p.18 / Chapter b) --- Proteoglycan --- p.19 / Chapter 2.6.2.3 --- Other differentially expressed factors --- p.20 / Chapter 2.7 --- Treatment of hypertrophic scar --- p.21 / Chapter Part II --- Differential Display / Chapter 2.8 --- Current approaches for the studies of differential gene expression --- p.23 / Chapter 2.9 --- Comparison amongst different approaches --- p.23 / Chapter 2.10 --- The strategy of Differential Display RT-PCR (DDRT-PCR) --- p.24 / Chapter 2.11 --- The application of DDRT-PCR to identify differentially expressed genes --- p.26 / Chapter Chapter 3 --- Aims and Strategies --- p.27 / Chapter Chapter 4 --- Methods and Materials --- p.29 / Chapter 4.1 --- Materials --- p.29 / Chapter 4.2 --- Clinical specimen collection --- p.31 / Chapter 4.3 --- Primary explant culture --- p.31 / Chapter 4.4 --- Immunohistochemical staining --- p.32 / Chapter 4.5 --- Total RNA extraction --- p.32 / Chapter 4.6 --- DNase I digestion --- p.33 / Chapter 4.7 --- Differential display-RTPCR (DD-RTPCR) --- p.33 / Chapter 4.8 --- Polyacrylamide gel electrophoresis --- p.34 / Chapter 4.9 --- Reamplification of the differentially expressed fragments --- p.35 / Chapter 4.10 --- Molecular cloning of the DNA fragments --- p.35 / Chapter 4.11 --- Screening and miniprep of the plasmid DNA --- p.36 / Chapter 4.12 --- Cycle sequencing --- p.38 / Chapter 4.13 --- Data analysis --- p.38 / Chapter 4.14 --- RT-PCR --- p.39 / Chapter 4.15 --- Probe labeling by PCR with DIG-dUTP --- p.40 / Chapter 4.16 --- Southern blotting --- p.41 / Chapter Chapter5 --- p.42 / Chapter 5.1 --- Clinical Specimen --- p.42 / Chapter 5.2 --- Primary explant culture --- p.42 / Chapter 5.3 --- The total RNA extraction from the cultured fibroblast --- p.45 / Chapter 5.4 --- Differential display RT-PCR --- p.47 / Chapter 5.5 --- Reamplification of the DNA fragments --- p.49 / Chapter 5.6 --- Molecular cloning of the DNA fragment --- p.53 / Chapter 5.7 --- DNA sequencing of the inserts --- p.58 / Chapter 5.8 --- Analysis and identification of the DNA sequences --- p.62 / Chapter 5.9 --- Semi-quantitative RT-PCR analysis of the differentially expressed genes --- p.76 / Chapter Chapter6 --- p.87 / Chapter Part I --- Validity of the Findings / Chapter 6.1 --- The Limitation of Tissue Sampling --- p.87 / Chapter 6.2 --- Tissue Culture model --- p.88 / Chapter 6.3 --- Differential Display RT-PCR --- p.89 / Chapter 6.3.1 --- Identification of the differentially expressed genes --- p.89 / Chapter 6.3.2 --- Confirmation of the differentially expressed genes --- p.91 / Chapter 6.4 --- Technical difficulties and Limitations --- p.92 / Chapter 6.4.1 --- Sampling --- p.92 / Chapter 6.4.2 --- Primary tissue culture --- p.93 / Chapter Part II --- Significance and Future Studies / Chapter 6.5 --- Down-regulation of thrombospondin 1 (TSP 1) in the hypertrophic scar fibroblasts --- p.94 / Chapter 6.6 --- Biochemical and biological functions of TSP1 --- p.96 / Chapter 6.6.1 --- The biochemical functions of TSP1 --- p.96 / Chapter 6.6.2 --- The biochemical functions of TSP1 --- p.97 / Chapter 6.7 --- The role of TSP 1 in the pathogenesis of hypertrophic scar --- p.98 / Chapter 6.7.1 --- Down-regulation of TSP 1 may be responsible for the excessive microvessels in hypertrophic scar --- p.98 / Chapter 6.7.2 --- Down-regulation of TSP 1 may be responsible for the failure of the apoptosis of the fibroblasts in the hypertrophic scar --- p.101 / Chapter 6.8 --- Expression of TSP 1 during wound healing --- p.103 / Chapter 6.9 --- Expression of TSP 1 in hypertrophic scarring --- p.107 / Chapter 6.10 --- Cytochrome b561 and its biological function --- p.109 / Chapter 6.11 --- Future studies --- p.108 / Chapter 6.11.1 --- The expression of TSP 1 in hypertrophic scarring and normal wound healing --- p.108 / Chapter 6.11.2 --- The expression of cytochrome b561 --- p.109 / Chapter 6.11.3 --- A full scale study of differential display RT-PCR --- p.109 / References --- p.110 / Appendices --- p.121 / Chapter I --- The complete mRNA sequence of thrombospondin1 precursor --- p.121 / Chapter II --- The mRNA sequence of cytochrome b561 --- p.123
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322514 |
| Date | January 1998 |
| Contributors | Cheng, Chi Wa., Chinese University of Hong Kong Graduate School. Division of Surgical Sciences. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xv, 123 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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