Un Ag HA para el virus SLE, se obtuvo en células suspendidas "in vitro", incubadas en 37 grados C a PH 7 a 8 con un óptimo de 7,2. La fuente celular fue células provenientes del Sarcoma 180 TG, cosechado de ratones y resuspendidas en el mismo fluído ascítico inactivado a 56 grados C durante 30 minutos. El HA se obtuvo del 3ro. a 6to. día de inoculadas las células con un óptimo entre el 5to. y 6to. día. Otras fuentes de células, plaquetas bovinas y células de cerebro de ratón, fueron también positivas a estos pHs y temperaturas pero dieron títulos más bajos. Las restantes fuentes de células resultaron negativas. / A hemagglutinating antigen for SLE virus was obtained in cells suspended "in vitro" incubated at 37 C at pH 7,0 to 8,0 with the optimal 7,2. The source of cell was Sarcoma 180 TG harvested from mice, and resuspended in the same ascitic fluid after inactivation at 56 C during 30 minutes. HA was recorded from the third to sixth day after seeding with the optimal between fifth and sixth day. Other two sources of cells: bovine platelets and mouse brain cells were also positive at these pH's and temperature but lower titlers were observed other eight sources of cells were all negatives.
| Identifer | oai:union.ndltd.org:SEDICI/oai:sedici.unlp.edu.ar:10915/1490 |
| Date | January 1985 |
| Creators | Fernández, Ana Sofía |
| Contributors | Mettler, Norma E. |
| Source Sets | Universidad Nacional de La Plata, Sedici |
| Language | Spanish |
| Detected Language | Spanish |
| Type | Tesis, Tesis de doctorado |
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