Matrix metalloproteinases (MMP) are a large family of proteinases that
remodel extracellular matrix (ECM) component. Recent data suggest a role for
MMPs in a number of renal pathophysiologies, associated with an imbalance of ECM
syntesis and degradation, which may result in an accumulation of ECM molecules
and renal fibrosis. The aim of this study is to elucidate the role of pro and activated
MMP-2 and 9 in urine and renal tissue of healty and nephropatic dogs. Renal tissue
of 8 healty dogs and either renal tissue and urine of 9 nephropatic dogs was collected
and analize using zimographic method, which is been validated in this study. Either
MMPs zimographic bands were present in almost all samples. In particular, pro and
activated MMP-9 zimographic bands were poorly represent in renal tissue of healty
dogs, whereas were very represent in nephropatic dogs. Pro and activated MMP-2
was present in either tissue of healty and nephropatic dogs. In urine of nephropatic
dogs, pro and activated MMP-9 was more evident than MMP-2, but there was not
correlaction with renal tissue levels, therefore urine levels of MMPs have poorly
usefulness in diagnostic pratice. The values of Pro and activated MMP-9 in
nephropatic dogs were significantly higher compared with normal dogs (p < 0,05),
whereas there was not statistically meaningful for Pro and activated MMP-2. In
conclusion, in this study we have validated a zimographic method for renal tissue of
dogs and we have illustrated the changes in nephropatic dogs, which may be useful
for further study.
| Identifer | oai:union.ndltd.org:unibo.it/oai:amsdottorato.cib.unibo.it:737 |
| Date | 11 April 2008 |
| Creators | Mancini, Danilo <1976> |
| Contributors | Famigli Bergamini, Paolo |
| Publisher | Alma Mater Studiorum - Università di Bologna |
| Source Sets | Università di Bologna |
| Language | Italian |
| Detected Language | English |
| Type | Doctoral Thesis, PeerReviewed |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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