FundaÃÃo de Amparo à Pesquisa do Estado do Cearà / CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior / Buccal illnesses as the caries and the gingivitis if configure as one of the main problems of the public health in the whole world. Oral Microorganism, in particular the streptococcus mutans group, are related to the formation of bacterial biofilm and the recurrent illnesses of it. A reduction of the number of these microorganisms implies, therefore, in prevention and control of these illnesses. In this work three solutions had been tested: clorexidina hidro-etan?lica 0.12% (positive control) and solutions of Neem (Azadirachta Indica) and watery from the essential oil of the Rosemary pepper (lippia sidoiddes) 1,0 %. Eighty and one voluntary healthy had been randomizados in three experimental groups in accordance with the solution in test. Clinical examinations for research and quantification of periodontais indices had been carried through in the three groups. Samples of saliva had been harvested, diluted and inoculated (50?l) in plates I contend Agar MSB. All the plates had been incubated in conditions of microaerofilic under atmosphere of 5% Co2 for 48 hours. After these procedures, had been gotten the basal values and compared with the values carried through after one week of mouths washing with the solutions it has tested being carried through a quantitative evaluation in these indices as well as in the numbers of units formers colonies of microorganisms of the group mutans, considering the initial values (without the use of the solutions), with the use of the solutions has controlled positive (Clorexidina 0,12%), with the use of the solutions in test in during one week of use and later following clinical examinations thirty days later. The results had shown that the three you substantiate tested had been efficient in reducing in statistically significant way all the four indices evaluated (p < 0.05). The degree of discomfort and possible collateral effect of the solutions had been evaluated through a questionnaire. The results had shown that it did not have statistically significant difference, in 5% level, in the reduction effect enter the three solutions tested for streptococcus of the group mutans. The reduction of the number was also observed of props up physicians in the three subsequent weeks to the use of the solutions with use of the solutions above cited. The biggest degree of discomfort was proportionate for the hidro-etan?lica solution of Neem 20%. The told discomforts had been (in % of the total of answers): bad taste (clorexidina 15%, alecrim 20%, garlic 100%), heat (clorexidina 6%, rosemary 18%, Neem 82%), bad breath (clorexidina 2%, alecrim 8%, Neem 88%) and nausea (clorexidina 0%, alecrim 0%, Neem 70%). Of this form, it was possible to conclude that, even so the solution of Neem has presented effect undesirable, this solution as well as the one of essential oil of rosemary pepper can be used as alternative the clorexidina / Doencas bucais como a carie e a gengivite se configuram como um dos principais problemas da saude publica em todo o mundo. A microbiota oral, em particular os estreptococos do grupo mutans, estao intimamente relacionados a formacao do biofilme bacteriano e das doencas dele decorrentes. Uma reducao do numero destes microrganismos implica, portanto, em prevencao e controle destas doencas. Neste trabalho foram testadas tres solucoes: clorexidina a 0,12% (controle positivo) e solucoes hidro-etanilica de Neem (Azadirachta Indica) e aquosas a partir do oleo essencial do Alecrim pimenta (lippia sidoiddes) a 1,0 %. Oitenta e um voluntarios sadios foram randomizados em tres grupos experimentais de acordo com a solucao em teste. Exames clinicos para pesquisa e quantificacao de indices periodontais foram realizados nos tres grupos. Amostras de saliva foram colhidas, diluidas e inoculadas (50 μl) em placas contendo Agar MSB. Todas as placas foram incubadas em condicoes de microaerofilia sob atmosfera de 5% CO2 por 48 horas. Apos estes procedimentos, foram obtidos os valores basais e comparados com os valores realizados apos uma semana de bochechos com as solucoes teste sendo realizado uma avaliacao quantitativa nestes indices assim como nos numeros de unidades formadoras de colonias de microrganismos do grupo mutans, considerando os valores iniciais (sem a utilizacao das solucoes), com o uso das solucoes controle positivo (Clorexidina 0.12%), com o uso das solucoes em teste em durante uma semana de uso e posteriormente seguindo exames clinicos trinta dias depois. Os resultados mostraram que as tres substancias testadas foram eficazes em reduzir de maneira estatisticamente significante todos os quatro indices avaliados.( p<0,05). O grau de desconforto e possiveis efeitos colaterais das solucoes foram avaliados atraves de um questionario. Os resultados mostraram que nao houve diferenca estatisticamente significante, em nivel de 5%, no efeito de reducao entre as tres solucoes testadas para estreptococos do grupo mutans. Foi observada tamb?m a reducao do numero de escores clinicos nas tres semanas subsequentes ao uso das solucoes com uso das solucoes acima citadas. O maior grau de desconforto foi proporcionado pela solucao hidro-etanilica de Neem a 20%. Os desconfortos relatados foram (em % do total de respostas): gosto ruim (clorexidina 15%, alecrim 20%, alho 100%), ardencia (clorexidina 6%, alecrim 18%, Neem 82%), mau halito (clorexidina 2%, alecrim 8%, Neem 88%) e nausea (clorexidina 0%, alecrim 0%, Neem 70%). Desta forma, foi possivel concluir que, embora a solucao de Neem tenha apresentado efeitos indesejaveis, esta solucao bem como a de oleo essencial de alecrim pimenta podem ser utilizadas como alternativa a clorexidina
| Identifer | oai:union.ndltd.org:IBICT/oai:www.teses.ufc.br:181 |
| Date | 29 April 2005 |
| Creators | Marco Antonio Botelho Soares |
| Contributors | JÃrg Heukelbach, Cibele Barreto Mano de Carvalho, Henrique Clasen Sarparo |
| Publisher | Universidade Federal do CearÃ, Programa de PÃs-GraduaÃÃo em SaÃde PÃblica, UFC, BR |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFC, instname:Universidade Federal do Ceará, instacron:UFC |
| Rights | info:eu-repo/semantics/openAccess |
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