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Isolation and Characterization of a new thermotolerant pigment- producing microalga: Salt stress enhances pigment and oil biosynthesis in Coelastrella sp.F50

A new species of reddish-orange pigment-producing microalga was isolated from a shallow pond in tropical Taiwan. Morphological and molecular evidence including meridional ribs on the cell wall, pigment production, and 18S rDNA sequence analysis suggest that this microalga is a species in the genus Coelastrella. Salt stress accelerated biosynthesis of the reddish-orange pigments, and large quantity of oil accumulated as the cells stressed under nutrient deficiency. This microalga could
sustain 45 ¢XC for more than 8 hours indicated by the stability of its chlorophylls, which is a necessary trait for large scale outdoor cultivation using photobioreactors in tropical areas. The reddish-orange pigments could be separated into many fractions by HPLC, and signals from carotenoids were detected in a few fractions using NMR, suggesting these pigments may function as antioxidants among other roles.

Identiferoai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0822112-152637
Date22 August 2012
CreatorsHu, Che-Wei
ContributorsChing-Nen Nathan Chen, Lee-Feng Chien, Chih-Sheng Lin
PublisherNSYSU
Source SetsNSYSU Electronic Thesis and Dissertation Archive
LanguageEnglish
Detected LanguageEnglish
Typetext
Formatapplication/pdf
Sourcehttp://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0822112-152637
Rightsuser_define, Copyright information available at source archive

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