O colágeno é a proteína mais abundante em mamíferos, sendo o tipo I a forma
prevalente. Essa proteína possui propriedades interessantes, como baixa
imunogenicidade e toxicidade, biocompatibilidade, além de promover o
reconhecimento, adesão e diferenciação celular através de sequências RGD
(arginina-glicina-aspartato). Por isso, o colágeno é utilizado na composição de
diversos biomateriais. Contudo, após a implantação in vivo, a degradação do material
por colagenases endógenas pode ocorrer rapidamente, limitando sua utilização. A
funcionalização com plasma frio pode superar essa limitação, sendo capaz de
adicionar uniformemente grupos funcionais na superfície de materiais e ser modulada
para preservar ou alterar de maneira seletiva as propriedades do material. Este estudo
buscou funcionalizar o pericárdio bovino (PB) – composto principalmente por colágeno
tipo I – utilizando plasma frio a fim de obter um novo biomaterial. Para isso, amostras
de PB foram tratadas com plasma de argônio e CO2 a 2,45GHz e 500 W em um
intervalo tempo dede 0s (PBCT) a 240s (PB240) com incrementos de 30s. As
amostras foram analisadas por espectroscopia de infravermelho e a validação da
análise foi realizada através de modelagem molecular computacional. O material foi
caracterizado por microscopia óptica e eletrônica de varredura, ângulo de contato e
capacidade higroscópica. A biocompatibilidade do PB tratado foi verificada pela
implantação do material na camada subcutânea de camundongos Balb/c durante 3, 7
15 e 30 dias. Os resultados mostraram que as modificações causadas pelo tratamento
com plasma foram tempo dependente. Após 120s de tratamento (PB120) houve a
adição de grupos éster na forma de ligações crosslink sem alterações à estrutura
proteica. Entretanto, após 150s foi observado o início de um processo de etching e
degradação do colágeno, acentuado aos 240s. Além disso, o tratamento provocou um
aumento na hidrofobicidade da superfície do material. A implantação subcutânea
mostrou que não houve perda na biocompatibilidade do nos grupos tratados quando
comparado ao controle. Dessa forma, verificou-se que o tratamento com plasma pode
ser modulado para modificar o colágeno sem que haja perdas em suas propriedades
de interesse. Novos estudos são necessários para a adequação da funcionalização
com plasma, direcionando-a para modificações desejadas e aplicações específicas
do biomaterial. / Collagen is the most commonly found protein in mammals and Type I is its most
prevalent type. This protein shows interesting properties as low immunogenicity and
toxicity, biocompatibility, besides the ability to promote cell recognition, differentiation
and induce cell attachment through RGD (arginine-glycine-aspartate) sequences. Due
to these characteristics, collagen is used in many biomaterials. However, after in vivo
implantation, the degradation of the material by endogenous collagenases can occur
too soon, impairing its application. Cold plasma functionalization can overcome this
limitation; it is able to add evenly new functional groups on materials’ surfaces and can
be tailored to preserve or alter selectively the bulk properties of the material. Therefore,
this study aimed the functionalization of bovine pericardium (PB) – material composed
of collagen type I – using cold plasma in order to obtain a new biomaterial. PB was
treated with argon (Ar) and carbon dioxide (CO2), microwave power source 2.45Hz,
500 W. The treatment was carried out from 0 (PBCT) up to 240s (PB240), with a 30s
increment. The samples were analysed by FTIR-ATR and the analysis validation was
done through computing molecular modelling. The material was characterized by
optical microscopy, SEM, contact angle and swelling. PB biocompatibility was
assessed through subcutaneous implantation of the material in mice Balb/c for 3, 7, 15
and 30 days. The results indicated the changes caused by plasma treatment were
time-dependent. By 120s of treatment (PB120) ester groups were added in the
material, forming crosslink bonds without modification within the protein structure while
150s of treatment caused the beginning of an etching process and collagen
degradation, which was more distinct at 240s; there was also an increase in the
hydrophobicity on the surface of the treated material. The subcutaneous implantation
test showed there wasn’t biocompatibility loss of treated PB when compared to control.
Therefore, it was verified that plasma treatment can be tailored to modify collagen
without impairing its properties of interest. New studies are necessary to suit plasma
functionalization according the desired modifications and specific applications of the
biomaterial. / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:tede/605 |
| Date | 22 November 2018 |
| Creators | BALTAZAR, Daniela Rubio |
| Contributors | ROCHA, Lenaldo Branco, 58861726534, http://lattes.cnpq.br/0054167120508364 |
| Publisher | Universidade Federal do Triângulo Mineiro, Instituto de Ciências Biológicas e Naturais - ICBN, Brasil, UFTM, Programa de Interdisciplinar em Biociências Aplicadas |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | Portuguese |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFTM, instname:Universidade Federal do Triangulo Mineiro, instacron:UFTM |
| Rights | http://creativecommons.org/licenses/by-nc-nd/4.0/, info:eu-repo/semantics/openAccess |
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