<p>In the year of 2020, an
international pandemic caused by severe acute respiratory syndrome coronavirus
2 (SARS-CoV-2) has afflicted tens of millions of people’s life also disrupting global
economics. Diagnostic testing is an important part of ensuring public health
until a vaccine that has been shown to be safe and effective is made available
to the general public. Most tests for detecting COVID-19 utilize quantitative
polymerase chain reaction (qPCR) assays, which is a specific and relatively
simple quantitative assay that could provide adequate sensitivity for
diagnosing early infection. Although powerful, these lab-based molecular assays
have a significant lag time, usually several days before receiving results. To
satisfy the needs of different purposes (diagnostics, screening, and
surveillance),
a unified approach is impractical. This thesis presents an alternative testing
method supporting the current procedure of point of care (POC) testing and in
community testing. This paper-based test overcomes the limitations of current
testing methods by utilizing reverse-transcription loop-mediated isothermal amplification
(RT-LAMP) and receiving the result on-site by a color change in the presence of
the virus within 60 minutes. The test utilizes untreated freshly collected
saliva, a less invasive specimen, as the sample and possesses a limit of
detection (LoD) of 200 copies of virus per microliter of whole saliva with an analytical
sensitivity of 97% and analytical specificity of 100%. The test requires
minimal operator training and could be fabricated on a large-scale using
roll-to-roll methods. Since the test is based on nucleic acids, the testing
platform itself lends to further applications <a>including
food safety monitoring, animal diagnostic, etc. simply by changing the specific
primers</a>. </p>
| Identifer | oai:union.ndltd.org:purdue.edu/oai:figshare.com:article/14515569 |
| Date | 29 April 2021 |
| Creators | Jiangshan Wang (10725807) |
| Source Sets | Purdue University |
| Detected Language | English |
| Type | Text, Thesis |
| Rights | CC BY 4.0 |
| Relation | https://figshare.com/articles/thesis/A_RAPID_PAPER-BASED_COLORIMETRIC_MOLECULAR_TEST_FOR_SARS-COV-2_POINT-OF-CARE_DIAGNOSTIC/14515569 |
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