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Previous issue date: 2014-12-16 / CAPES / Pteridium (aquilinum) arachnoideum is a cosmopolitan plant, responsible for heavy losses in cattle for heavy losses in almost all Brazil. This plant causes three clinical pictures: an acute disease, characterized by hemorrhages and fever, and two chronic diseases characterized by bladder tumors and carcinomas of the superior digestive tract. In Santa Catarina, in a retrospective study, the acute poisoning is the form that prevailed. Most of the cases ocurred in the autumn and the main clinical signs observed were fever and faeces with blood. The macroscopic lesions were widespread hemorrhages and infarcts mainly in lung, liver, intestine and lymphnodes, which were characterized by necrosis associated with groups of basophylic bact?ria, sometimes with formation of bubbles of gas. In the bone marrow there was rarefaction or absence of hematopoietic tissue. The experimental reproduction of the disease was realized in four cattle, two vaccinated against clostridioses and two not vaccinated. These cattle received doses of 20, 20, 14 and 10g/kg/day, and they died after 82, 94, 46 and 76 days, when they had ingested 149, 180, 71 and 75% of the plant in relation to their weight. The course of the clinical signs was 5, 4, 1 and 5 days, and at post-mortem examination the lesions found were hemorrhages of varied degrees and locations. Liver infarcts were found in bovines 2, 3 and 4, and in the intestine in all the cattle. The histological lesions were characterized by rarefaction and absence of hematopoietic tissue in the bone marrow, necrosis and bacterial aggregates in the liver, lung, intestine and lymphnodes. Histopathology did not reveal inflammatory reaction and if present the intensity was slight. Samples of organs with infarcts collected at necropsy, in the spontaneous and experimental intoxication, sown in Tarozzi medium, produced gas. Five samples caused death in mice after inoculation of the medium. At necropsy the carcasses had putrid smell, the subcutaneous tissue was red and there was edema and red liquid in the abdominal cavity. Two mice that were sacrificed presented inflammatory reaction in the place of the application, characterized by areas of adherence of the skin to the subcutaneous tissue and presence of abscesso, and four presented putrid smell at necropsy. The impression of of the liver capsula of the mice that died or got sick, revealed small Gram positive rods. Histopathology of the mice that died, revealed in the skeletal musculature of the thighs edema between the fibers with necrosis and eosinofilia of fibers and great amount of small basophylic rods, associated with slight inflammatory mononuclear infiltration and hemorrhage. In the skin also inflammatory filtrate was observed, with edema in the derma and great amount of small basophylics rods. The rest of the mice were sacrificed and no alterations were found. The identification by Chain reaction of Polimerase (PCR) of the liver of the mice that had died, in Tarozzi medium, resulted in Clostridium septicum. / Pteridium (aquilinum) arachnoideum ? planta cosmopolita, respons?vel por perdas vultuosas na cria??o de bovinos em quase todas as regi?es do Brasil. Esta planta ? respons?vel por causar tr?s quadros cl?nicos: um quadro agudo, caracterizado por hemorragias e febre, e dois quadros cr?nicos caracterizados por tumores de bexiga e do trato digest?rio superior. Em Santa Catarina, em estudo retrospectivo, a intoxica??o aguda ? a forma que prevaleceu sobre as demais. A maioria dos casos ocorreu no outono e os principais sinais cl?nicos observados foram febre e fezes com sangue. As les?es macrosc?picas encontradas foram hemorragias generalizadas e infartos principalmente em pulm?o, f?gado, intestino e linfonodo, os quais se caracterizavam por necrose associada a agregados bacterianos bas?filos, em alguns casos com forma??o de bolhas de g?s. Na medula ?ssea havia rarefa??o ou aus?ncia do tecido hematopo?tico. A reprodu??o experimental da doen?a foi realizada em quatro bovinos, dois vacinados contra clostridioses e dois n?o vacinados. Estes receberam doses de 20, 20, 14 e 10g/kg/dia de Pteridium (aquilinum) arachnoideum, e morreram com 82, 94, 46 dias e 76 dias, quando tinham ingerido 149, 180, 71 e 75% de planta em rela??o ao peso vivo. A evolu??o dos sinais cl?nicos foi de 5, 4, 1 e 5 dias, e, ? necropsia, as les?es consistiram de hemorragias em variados graus e localiza??es. Infartos de f?gado foram encontrados nos Bovinos 2, 3 e 4 e no intestino em todos os bovinos. As les?es histol?gicas se caracterizaram por rarefa??o e aus?ncia de tecido hematopo?tico na medula ?ssea, necrose e agregados bacterianos no f?gado, pulm?o, intestino e linfonodo. As les?es histol?gicas n?o revelaram rea??o inflamat?ria e quando presente, a intensidade era leve. Amostras de ?rg?os com infartos coletadas de necropsias nas intoxica??es espont?nea e experimental foram semeadas no meio de cultivo de Tarozzi e produziram g?s. Cinco amostras causaram a morte dos camundongos ap?s inocula??o do meio. ? necropsia desses camundongos verificou-se carca?as com cheiro p?trido, tecido subcut?neo avermelhado e com edema e l?quido avermelhado na cavidade abdominal. Dois camundongos que foram eutanasiados apresentaram rea??o inflamat?ria no local da aplica??o, caracterizada por ?reas de ader?ncia da pele com o tecido subcut?neo e abscessos; quatro exalavam cheiro p?trido na hora da realiza??o da necropsia. A impress?o da c?psula do f?gado dos camundongos que morreram e que ficaram doentes, revelou bastonetes Gram-positivos. ? histologia dos camundongos que morreram verificou-se na musculatura esquel?tica da regi?o da coxa, edema e hemorragia entre as fibras, necrose e eosinofilia de fibras e grande quantidade de bastonetes bas?filos, associado a infiltrado inflamat?rio mononuclear leve e hemorragia. Na pele tamb?m verificou-se, infiltrado inflamat?rio, com edema na derme e grande quantidade de bastonetes bas?filos. Os demais camundongos foram eutanasiados e n?o tiveram altera??es. A identifica??o por Rea??o em Cadeia de Polimerase (PCR) do meio de Tarozzi do f?gado dos camundongos que morreram foi detectado Clostridium septicum.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/2306 |
| Date | 16 December 2014 |
| Creators | J?NCK, Fernanda |
| Contributors | Tokarnia, Carlos Maria Antonio Hubinger, Tokarnia, Carlos Maria Antonio Hubinger, Gava, Aldo, Dutra, Iveraldo dos Santos, Queiroz, Marilene de Farias Brito, D?bereiiner, J?rgen |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Medicina Veterin?ria (Patologia e Ci?ncias Cl?nicas), UFRRJ, Brasil, Instituto de Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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