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Suppression of peptide ions dissociation under electron capture condition.

Wong, Pui Shuen. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 92-96). / Abstracts in English and Chinese. / Title Page --- p.i / Abstract (English) --- p.ii / Abstract (Chinese) --- p.iii / Acknowledgements --- p.iv / Table of Contents --- p.V / List of Tables --- p.viii / List of Figures --- p.ix / List of Schemes --- p.xi / Symbols and Abbreviations --- p.xii / Dedication --- p.xiv / Chapter Chapter 1 --- Introduction / Chapter 1.1 --- Mass Spectrometry of peptides/ proteins --- p.1 / Chapter 1.1.1 --- Electrospray ionization of peptides/ proteins --- p.3 / Chapter 1.2 --- Tandem mass spectrometry of peptides/ proteins --- p.4 / Chapter 1.2.1 --- Nomenclature of peptide fragment ions --- p.5 / Chapter 1.2.2 --- Slow heating methods for MSn --- p.6 / Chapter 1.2.2.1 --- Collision induced dissociation --- p.7 / Chapter 1.2.3 --- "Electron based ion activation for MS""" --- p.8 / Chapter 1.3 --- Electron Capture Dissociation --- p.9 / Chapter 1.3.1 --- ECD mechanism for protonated peptide ions --- p.10 / Chapter 1.3.2 --- ECD efficiency --- p.12 / Chapter 1.3.3 --- ECD of metal ions-adducted peptide --- p.13 / Chapter 1.4 --- Overview of present work --- p.14 / Chapter Chapter 2 --- "Instrumentation, Experimental and Calculations" / Chapter 2.1 --- Fourier-transform Ion Cyclotron Resonance Mass Spectrometer --- p.15 / Chapter 2.1.1 --- Basic principle of FTICR-MS --- p.15 / Chapter 2.1.2 --- The instrument --- p.19 / Chapter 2.1.2.1 --- Vacuum system --- p.19 / Chapter 2.1.2.2 --- Nanospray source --- p.24 / Chapter 2.1.2.3 --- Electrostatic ion focusing system --- p.26 / Chapter 2.1.2.4 --- Infinity´ёØ Cell --- p.28 / Chapter 2.1.2.5 --- Electron emission source --- p.29 / Chapter 2.1.2.6 --- Data acquisition system --- p.31 / Chapter 2.2 --- Experimental --- p.31 / Chapter 2.2.1 --- Acquisition pulse program --- p.31 / Chapter 2.2.1.1 --- Simple ESI acquisition pulse program (MS experiment) --- p.31 / Chapter 2.2.1.2 --- ESI-ECD acquisition pulse program (MS experiment) --- p.34 / Chapter 2.2.2 --- Molecular mechanics calculation --- p.36 / Chapter Chapter 3 --- Structural Parameters Affecting Suppression of N-Ca Cleavages of Peptides Ions after Electron Capture / Chapter 3.1 --- Introduction --- p.37 / Chapter 3.2 --- Experimental section --- p.39 / Chapter 3.3 --- Results and Discussion --- p.40 / Chapter 3.3.1 --- Peptides with three arginine residues --- p.40 / Chapter 3.3.1.1 --- General ECD mass spectra features --- p.40 / Chapter 3.3.1.2 --- Comparison of the extent of suppression of triariginated and diarginated model peptides ions --- p.46 / Chapter 3.3.2 --- Peptides with histidine and lysine as proton carriers --- p.47 / Chapter 3.3.2.1 --- General features of ECD mass spectra --- p.47 / Chapter 3.3.2.2 --- Comparison of ECD behavior of peptide ions with different proton carrier --- p.50 / Chapter 3.3.3 --- Peptides with various chain length --- p.53 / Chapter 3.3.3.1 --- General ECD mass spectra features --- p.53 / Chapter 3.3.3.2 --- Reactivation of [M+2H]+* by collision activation --- p.57 / Chapter 3.3.3.3 --- Significance of glutamic acid residues --- p.57 / Chapter 3.3.3.4 --- Results of conformational searches --- p.60 / Chapter 3.4 --- Conclusions --- p.64 / Chapter Chapter 4 --- Investigation of the Role of Conformation of Peptide Ions in Suppression of Backbone fragmentation / Chapter 4.1 --- Introduction --- p.67 / Chapter 4.2 --- Experimental section --- p.69 / Chapter 4.3 --- Results and Discussion --- p.70 / Chapter 4.3.1 --- Peptide with N-methylated amino acid residues --- p.70 / Chapter 4.3.1.1 --- General features of ECD mass spectra --- p.70 / Chapter 4.3.1.2 --- Comparison between normal and N-methylated peptide ions under ECD --- p.72 / Chapter 4.3.2 --- Peptides with proline residues vi --- p.73 / Chapter 4.3.2.1 --- General ECD mass spectra features --- p.73 / Chapter 4.3.2.2 --- Comparison of ECD of peptide ions with and without proline residues --- p.76 / Chapter 4.3.3 --- Transition metal ions as charge carriers --- p.80 / Chapter 4.3.3.1 --- General ECD mass spectra features --- p.80 / Chapter 4.3.3.2 --- Comparison of ECD behavior using proton and metal ions as charge carrier --- p.85 / Chapter 4.4 --- Conclusions --- p.87 / Chapter Chapter 5 --- Conclusions --- p.90 / References --- p.92 / Chapter Appendix I --- Twenty common amino acids --- p.97 / Chapter Appendix II --- Pulse programs for MS and MSn experiments --- p.98

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_327354
Date January 2011
ContributorsWong, Pui Shuen., Chinese University of Hong Kong Graduate School. Division of Chemistry.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xiv, 105 leaves : ill. (some col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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