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Previous issue date: 2013-04-30 / Dietary habits composed of high levels of fat are a trigger for obesity. This pandemic is involved with the development of cardiovascular diseases, due to structural and hemodynamic changes in the heart. The G-CSF has emerged as a potential treatment for many degenerative diseases, emphasizing its beneficial effect on the structural and functional regeneration of the heart in ischemic cardiomyopathy and Chagas disease, with diminished inflammation and fibrosis, and improvement of cardiac impulse generation and conduction. We used C57BL / 6 mice fed a high-fat (HF) diet that developed obesity, hyperglycemia and glucose intolerance. The changes in cardiac function were assessed by electrocardiogram, echocardiogram and treadmill. The animals were divided into two groups: standard diet (n = 8) and HF diet (n = 16) to induce obesity. 37 weeks after HF protocol, the animals were divided into three groups: treated with G-CSF (n = 8) + standard diet, not treated with G-CSF (n = 8) + standard diet, and the third group with standard diet only (n = 8). Results showed that replacing the standard diet following HF diet was responsible for the return to baseline of anthropometric and biochemical values, which were significantly elevated. Diet replacement alone did not reverse exercise intolerance or prevent cardiac abnormalities, such as bradycardia and increases in QRS amplitude, septal wall thickness and left ventricular posterior thickness. Diet replacement and treatment with G-CSF reversed structural changes in the heart and recovery of physical capacity. / H?bitos alimentares de altos teores de gordura s?o fatores desencadeantes da obesidade. Essa pandemia est? envolvida com o desenvolvimento de doen?as cardiovasculares, devido a altera??es estruturais e hemodin?micas no cora??o. O G-CSF surge como um tratamento em potencial para a terapia de diversas doen?as degenerativas, destacando-se seu efeito ben?fico na regenera??o estrutural e funcional do cora??o nas cardiomiopatias isqu?mica e chag?sica cr?nica com diminui??o de inflama??o e fibrose, e melhora na gera??o e condu??o do impulso card?aco. Utilizou-se camundongos C57Bl/6 machos alimentados com dieta hipergordurosa (high-fat HF) que desenvolveram obesidade, hiperglicemia e intoler?ncia ? glicose. As altera??es funcionais card?acas foram avaliadas por eletrocardiograma, ecocardiograma e ergometria. Os animais foram separados em 2 grupos experimentais: dieta padr?o (n=8) e dieta HF (n = 16) para indu??o da obesidade. 37 semanas ap?s a introdu??o da HF, os animais foram separados em 3 grupos: tratado com G-CSF (n=8) + dieta padr?o; n?o tratado com G-CSF (n=8) + dieta padr?o, e o terceiro grupo somente com dieta padr?o (n=8). Resultados mostraram que a substitui??o da dieta HF pela dieta padr?o foi respons?vel pelo retorno ao n?vel basal dos valores bioqu?micos e antropom?tricos, que apresentavam-se elevados. Apenas a substitui??o da dieta n?o reverteu ? intoler?ncia ao exerc?cio nem preveniu as altera??es card?acas, como bradicardia, aumento da amplitude de QRS e aumento da espessura das paredes septal e posterior do ventr?culo esquerdo. A substitui??o da dieta e o tratamento com G-CSF reverteram as altera??es estruturais do cora??o e recuperarem a capacidade f?sica.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.uefs.br:8080:tede/264 |
| Date | 30 April 2013 |
| Creators | Daltro, Pamela Santana |
| Contributors | Macambira, Simone Garcia |
| Publisher | Universidade Estadual de Feira de Santana, Mestrado Acad?mico em Biotecnologia, UEFS, Brasil, DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UEFS, instname:Universidade Estadual de Feira de Santana, instacron:UEFS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 7701973706309601282, 600, 600, 600, -6971480722008537872, -1634559385931244697 |
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