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Previous issue date: 2012-02-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Periodontal disease is a complex inflammatory and infectious condition that immune host, front of the microbial aggressions, can lead to disease progression, resulting in tissue destruction. The tissue damage induces the release of regulatory molecules, which protective roles and / or destructive, including proteins VEGF (vascular endothelial growth factor vascular) and HIF-1 ? (hypoxia-induced factor ? -1). Thus, this study investigated,
quantitatively and comparatively, the immunohistochemical expression of VEGF (vascular endothelial growth factor) and HIF-1 ? (hypoxia induced factor 1-?), proteins involved in
inflammation, angiogenesis and hypoxia, in human gingival tissues. Therefore, 75 samples of gingival tissues were examined. Thirty samples were chronic periodontitis, 30 with chronic
gingivitis and 15 healthy gingival. After sections analysis, positives and negatives inflammatory and endothelial cells in the connective tissue were counted and converted into
percentage. Data were statistically analyzed using Kruskal-Wallis test and Spearman correlation. The results showed that both proteins marked. It was observed higher immunoreactivity for HIF-1 ? at chronic gingivitis and periodontitis specimens compared to healthy sites, however, no statistically significant differences were observed among them (p>0.05). The VEGF immunostaining showed similarity among the cases of periodontitis, gingivitis and healthy gingiva. Moderate and positive correlation statistically significant differences were verified for the expressions of VEGF and HIF-1? in gingival health (r = 0,529, p = 0.04). Thus, it can be conclude that possibly the route of HIF-1 ?, is activated in periodontal disease may have involvement of the protein in pathogenesis and progression of
disease, and that activation of VEGF, can be in addition to being triggered transcription by HIF-1 ? may be also influenced by other additional factors such as the action of periodontal
microorganisms endotoxins / A doen?a periodontal ? uma condi??o infecciosa e inflamat?ria complexa em que a resposta imune do hospedeiro, frente aos produtos microbianos, pode conduzir ? progress?o da doen?a. A agress?o tecidual induz a libera??o de mol?culas reguladoras, que desempenham pap?is protetores e/ou destrutivos, incluindo as prote?nas VEGF (Fator de crescimento endotelial vascular) e o HIF-1 ? (Fator induzido por hip?xia -1 ?). Diante disso, este estudo buscou analisar, de forma quantitativa e comparativa, a express?o imuno-histoqu?mica destas prote?nas, envolvidas nos processos de angiog?nese e hip?xia, observadas em condi??es
inflamat?rias. Para tanto, 75 amostras de tecidos gengivais foram examinadas. Destas, 30 foram de periodontite cr?nica, 30 de gengivite cr?nica e 15 de gengivais saud?veis. Foram
contadas, as c?lulas inflamat?rias e endoteliais, positivas e negativas, no tecido conjuntivo fibroso, e posteriormente, convertidas em porcentagem. Os dados foram analisados
estatisticamente por meio do teste de Kruskal-Wallis e Correla??o de Spearman. Os resultados mostraram imunoexpress?o para ambas as prote?nas. Foi observada, uma elevada imunoexpress?o para HIF-1 ?, nos esp?cimes de periodontite e gengivites cr?nicas, em rela??o aos s?tios saud?veis, por?m, sem diferen?as estat?sticas entre elas (p>0,05). A imunoexpress?o do VEGF demonstrou similaridade, entre os casos de periodontite, gengivite e gengiva saud?vel. Correla??o positiva moderada e diferen?a estatisticamente significativa foram verificadas para as express?es do VEGF e HIF-1?, em gengivais saud?veis (r=0.529; p= 0.04). Desta forma, pode-se concluir que, possivelmente a via de ativa??o do HIF-1 ?, est? ativada na doen?a periodontal, podendo haver participa??o desta prote?na, na patog?nese e progress?o da doen?a periodontal e que a ativa??o do VEGF, al?m de ser desencadeada via transcri??o do HIF-1 ?, pode ser tamb?m, influenciada por outros fatores adicionais, como, por exemplo, a??o das endotoxinas bacterianas periodontopatog?nicas
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/17119 |
| Date | 28 February 2012 |
| Creators | Vasconcelos, Roseane Carvalho |
| Contributors | CPF:02569549410, http://lattes.cnpq.br/4649601602612601, Lemos, Jana?na Cavalcante, CPF:77846532472, http://lattes.cnpq.br/6756263467790240, Queiroz, L?lia Maria Guedes, CPF:59539224420, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793681A0&dataRevisao=null, Gurgel, Bruno C?sar de Vasconcelos |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Patologia Oral, UFRN, BR, Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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