Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-07-11T17:13:31Z No. of bitstreams: 1 PatriciaGuerraPeixeGoncalves_DISSERT.pdf: 943209 bytes, checksum: 858cf53a3d375685e3e69be1d38caba2 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-07-15T20:21:04Z (GMT) No. of bitstreams: 1 PatriciaGuerraPeixeGoncalves_DISSERT.pdf: 943209 bytes, checksum: 858cf53a3d375685e3e69be1d38caba2 (MD5) / Made available in DSpace on 2016-07-15T20:21:04Z (GMT). No. of bitstreams: 1 PatriciaGuerraPeixeGoncalves_DISSERT.pdf: 943209 bytes, checksum: 858cf53a3d375685e3e69be1d38caba2 (MD5) Previous issue date: 2016-02-22 / A angiog?nese e a linfangiog?nese s?o altera??es tamb?m decorrentes da inflama??o gengival provocada por microrganismos presentes no biofilme dental, bem como pela a migra??o de c?lulas de defesa e secre??o de mediadores inflamat?rios no local da agress?o. Este estudo teve por objetivo avaliar a angiog?nese e linfangiog?nese em 90 esp?cimes de bi?psias de tecido gengival clinicamente saud?vel, com gengivite e com periodontite cr?nicas. Os cortes histol?gicos foram avaliados pela colora??o de hematoxilina e eosina e pela t?cnica de imunoistoqu?mica atrav?s da imunomarca??o de CD34 e podoplanina, para avaliar, respectivamente, o ?ndice angiog?nico e linfangiog?nico, por meio da t?cnica de contagem microvascular. Os resultados mostraram que h? correla??o entre os ?ndices (p=0,030), por?m, mostrou que na periodontite h? menos n?meros de vasos linf?ticos do que no tecido gengival clinicamente saud?vel (p=0,016). A podoplanina mostrou marca??o no epit?lio e que h? rela??o da intensidade de marca??o com a intensidade do infiltrado inflamat?rio, sendo mais intensa a marca??o na presen?a de infiltrado inflamat?rio severo (p=0,033). Concluiu-se neste estudo que h? menor n?mero de vasos sangu?neos na periodontite em compara??o com a gengiva clinicamente saud?vel. As sinaliza??es presentes no processo inflamat?rio, bem como o real papel da vasculatura sangu?nea e linf?tica gengival ainda n?o est?o totalmente elucidadas / Angiogenesis and lymphangiogenesis are changes that occur due to gingival inflammation caused by microorganisms present in the biofilm, as well as the migration of immune cells and secretion of mediators in the aggressed site. This study aimed to research angiogenesis and lymphangiogenesis in 90 specimens of clinically healthy, with gingivitis and chronic periodontitis gingival tissue biopsies. The histological sections were evaluated by hematoxylin and eosin and the immunohistochemical technique through immunostaining for CD34 and podoplanin. To evaluate the angiogenic and lymphangiogenic indexes we performed a microvessel counting technique. The results showed that there is a correlation between the indexes (p = 0.030), however, we observed that periodontitis showed less lymphatic vessels than clinically healthy gingival tissue (p = 0.016). Podoplanin showed positive staining in the basal layers of the epithelium, and we observed a relationship between immunostaining intensity and the intensity of inflammatory infiltrate, with more intense staining in the presence of severe inflammatory infiltrate (p = 0.033). For this study, we concluded that there are fewer blood vessels in periodontitis compared with clinically healthy gingiva. The signaling present in the inflammatory process and the actual role of gingival blood and lymphatic vasculature are not fully understood, with further studies on angiogenesis and lymphangiogenesis being suggested.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-04-03T23:22:20Z No. of bitstreams: 1 MarianaLinharesAlmeida_DISSERT.pdf: 1889702 bytes, checksum: ef1e5b69f2c491bf3e68bbe58a250218 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-04-12T18:32:53Z (GMT) No. of bitstreams: 1 MarianaLinharesAlmeida_DISSERT.pdf: 1889702 bytes, checksum: ef1e5b69f2c491bf3e68bbe58a250218 (MD5) / Made available in DSpace on 2017-04-12T18:32:53Z (GMT). No. of bitstreams: 1 MarianaLinharesAlmeida_DISSERT.pdf: 1889702 bytes, checksum: ef1e5b69f2c491bf3e68bbe58a250218 (MD5) Previous issue date: 2017-02-23 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / A rela??o entre a doen?a periodontal (DP) e a diabetes mellitus (DM) ? bidirecional, por?m, existe a necessidade de maiores evid?ncias em rela??o ao tratamento periodontal b?sico sobre os aspectos cl?nicos e a presen?a de bact?rias em pacientes com e sem diabetes. Dessa forma, portanto, o presente estudo teve por objetivo avaliar a influ?ncia da diabetes mellitus tipo II na efic?cia do tratamento periodontal b?sico atrav?s dos par?metros cl?nicos e microbiol?gicos da saliva. Foram tratados 51 pacientes entre 30 e 65 anos atrav?s da t?cnica ?Full Mouth Disinfection? (FMD), sendo 17 do grupo diab?tico (GD) e grupo n?o diab?tico (GND). Esses pacientes foram avaliados antes do in?cio do tratamento periodontal, aos 6 e 12 meses ap?s a sua conclus?o. No exame periodontal foram avaliados o ?ndice de Sangramento a Sondagem (ISS), ?ndice de Placa Vis?vel (IPV), Profundidade de Sondagem (PS), Recess?o Gengival (RG) e N?vel Cl?nico de Inser??o (NCI). Os par?metros sangu?neos avaliados foram glicose em jejum e hemoglobina glicada (HbA1C) apenas para o GD. As amostras de saliva foram coletadas antes da realiza??o de cada exame periodontal. Foi realizada a quantifica??o das esp?cies bacterianas Treponema denticola, Porphyromonas gingivalis, e Tannerella forsythia atrav?s da t?cnica Rea??o em cadeia da pol?meras em tempo real (PCR real-time). Os resultados mostraram quanto aos par?metros cl?nicos que houve uma melhora significativa para ISS, IPV e PS para ambos os grupos, enquanto que para o NCI apenas para o GD (p=0,023). Em rela??o aos par?metros microbiol?gicos, n?o houve diferen?as entre o grupo com diabetes e sem diabetes nos per?odos avaliados, apenas a esp?cie Tanerella forsythia apresentou diferen?a estat?stica significativa no per?odo de 12 meses (p=0,004), com maior mediana para o grupo GND. Na an?lise intragrupo, apenas o GD apresentou diferen?a estat?stica para todas as esp?cies avaliadas (p<0,05), na qual houve redu??o ap?s o tratamento, mas aumento ap?s a avalia??o de 6 meses. A esp?cie Porphyromonas gingivalis aumentou significativamente ap?s 12 meses, nesse per?odo de avalia??o obteve mediana maior que o baseline. A diabetes n?o influenciou na resposta do tratamento periodontal, ambos os grupos apresentaram melhora dos par?metros cl?nicos avaliados. / The relationship between periodontal disease (PD) and diabetes mellitus (DM) is bidirectional, however, there is a need for more evidence regarding basic periodontal treatment on clinical aspects and the presence of bacteria in patients with and without diabetes. Thus, the present study aimed to evaluate the influence of type II diabetes mellitus (DM) on the efficacy of basic periodontal treatment through the clinical and microbiological parameters of saliva. Fifty-one patients were treated between 30 and 65 years of age using the "Full Mouth Disinfection" (FMD) technique, 17 in the diabetic group (DG) and non-diabetic group (NDG). These patients were evaluated before the beginning of the periodontal treatment, at 6 and 12 months after its conclusion. In the periodontal examination, the Bleeding on Probing (BOP), Visible Plaque Index (VPI), Probing Depth (PD), Gingival Recession (RG) and Clinical Attachment Level (CAL) were evaluated. The blood parameters evaluated were fasting glucose and glycated hemoglobin (HbA1C) only for DG. Saliva samples were collected before each periodontal examination. Quantification of the bacterial species Treponema denticola, Porphyromonas gingivalis, and Tannerella forsythia through the real-time polymer chain reaction (PCR) technique was performed. The results showed that the clinical parameters showed a significant improvement for ISS, IPV and PS for both groups, whereas for NCI only for GD (p = 0.023). Regarding the microbiological parameters, there were no differences between the group with diabetes and no diabetes in the evaluated periods, only Tanerella forsythia presented a statistically significant difference in the 12-month period (p = 0.004), with a higher median for the NDD group. In the intragroup analysis, only the DG presented statistical difference for all species evaluated (p <0.05), in which there was reduction after treatment, but increase after the evaluation of 6 months. The species Porphyromonas gingivalis increased significantly after 12 months, in this period of evaluation it obtained median higher than baseline.

Made available in DSpace on 2015-04-14T14:50:59Z (GMT). No. of bitstreams: 1 418471.pdf: 941887 bytes, checksum: 83e65c46f62b9cf566ee10180ce3d11c (MD5) Previous issue date: 2009-03-19 / O receptor B&#8321; para as cininas est? normalmente ausente durante condi??es fisiol?gicas podendo ser rapidamente induzido em resposta a diversos est?mulos, como os processos infecciosos. Apesar disso, n?o existem estudos indicando como o LPS de Porphyromonas gingivalis (Pg-LPS), um ativador preferencial de TLR2, pode induzir a express?o do receptor B&#8321;. No presente trabalho, foi demonstrado que a inje??o do Pg-LPS na pata de rato resulta na indu??o funcional tempo-dependente dos receptores B&#8321; (determinada pelo aumento marcante na resposta edematog?nica), que foi precedida pelo r?pido aumento na express?o do seu RNAm. A administra??o local de Pg-LPS produziu um aumento marcante na produ??o da citocina pr?-inflamat?ria TNF-G e do influxo de neutr?filos. Ambos os eventos foram observados anteriormente ? indu??o dos receptores B&#8321;. A indu??o funcional e molecular do receptor B&#8321; pelo Pg-LPS foi reduzida significantemente pelo glicocortic?ide dexametasona e pelo anticorpo monoclonal anti- TNF-G infliximab. De grande relev?ncia, foi demonstrado pela primeira vez, que uma ?nica administra??o de Resolvina E1, um mediador lip?dico pr?-resolu??o da inflama??o, foi capaz de produzir uma redu??o marcante na express?o dos receptores B&#8321; mediada pelo Pg-LPS, provavelmente inibindo produ??o de TNF-G e a migra??o de neutr?filos para o s?tio inflamat?rio. Analisados em conjunto, os dados obtidos permitem inferir que a ativa??o do TLR2 pelo Pg-LPS ? capaz de induzir a express?o dos receptores B&#8321;, atrav?s de mecanismos relacionados ? produ??o de TNF-G e ao influxo de neutr?filos, que s?o sens?veis a Resolvina E1. Assim, ? poss?vel sugerir que os receptores B&#8321; representam uma via importante nas respostas inflamat?rias desencadeadas pela P. gingivalis e seus fatores de virul?ncia.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-02-29T22:55:50Z No. of bitstreams: 1 JoseNazarenoMoreiraDeAguiarJunior_TESE.pdf: 2810253 bytes, checksum: d577f52edf024bf1bd64c021a5bed745 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-03-02T19:49:24Z (GMT) No. of bitstreams: 1 JoseNazarenoMoreiraDeAguiarJunior_TESE.pdf: 2810253 bytes, checksum: d577f52edf024bf1bd64c021a5bed745 (MD5) / Made available in DSpace on 2016-03-02T19:49:24Z (GMT). No. of bitstreams: 1 JoseNazarenoMoreiraDeAguiarJunior_TESE.pdf: 2810253 bytes, checksum: d577f52edf024bf1bd64c021a5bed745 (MD5) Previous issue date: 2015-02-24 / A doen?a periodontal ? uma condi??o inflamat?ria cr?nica de car?ter infeccioso causada primariamente por bact?rias presentes em um biofilme dent?rio que interagem com o hospedeiro, determinando, assim, a natureza da doen?a resultante. Apesar de j? se conhecer muito sobre a patog?nese destas patologias, ainda n?o se sabe a composi??o exata do perfil de c?lulas T durante a fase ativa da doen?a (Th1, Th2 ou Th17). Este trabalho visou avaliar, atrav?s da express?o imuno-histoqu?mica, a presen?a dos marcadores (IL-17, IL-23 e ROR?t), envolvidos na resposta Th 17 em casos de gengiva clinicamente saud?veis (n=32), gengivite induzida pelo biofilme dental (n=30), periodontite cr?nica (n=32) e periodontite agressiva (n=25), objetivando analisar se a express?o e/ou distribui??o destas mol?culas em linf?citos e macr?fagos, presentes no infiltrado inflamat?rio dos tecidos periodontais, influencia na destrui??o tecidual observada nestas doen?as. Foi realizada a an?lise morfol?gica dos casos, onde avaliou-se a intensidade do infiltrado inflamat?rio em leve, moderado e intenso. Para cada caso, nas ?reas mais imunomarcadas, 5 campos foram escolhidos e analisados, tanto em rela??o a intensidade do infiltrado inflamat?rio quanto a quantidade de c?lulas imunomarcadas, baseando-se em escores predeterminados: escore 0 (aus?ncia de infiltrado inflamat?rio/imunomarca??o), escore 1 (o infiltrado/imunomarca??o abrangia menos de 25% da ?rea do campo), escore 2 (o infiltrado/imunomarca??o ocupava entre 25 e 50%) e escore 3 (infiltrado/imunomarca??o presente em mais de 50% da ?rea do campo). A partir disto, gerouse uma mediana que representava cada caso. A intensidade do infiltrado inflamat?rio foi correlacionada com a progress?o da doen?a, se mostrando crescente da gengiva clinicamente saud?vel at? a periodontite agressiva (p<0,001). Detectou-se a presen?a da IL-17, IL-23 e do ROR?t na maioria dos casos avaliados e a quantidade de c?lulas imunomarcadas foi correlacionada tanto com a intensidade do infiltrado inflamat?rio (P<0,001) quanto com os par?metros cl?nicos analisados (P<0,001), apresentando uma correla??o positiva, predominantemente moderada. A periodontite agressiva apresentou um maior percentual de imunomarca??o em rela??o ?s outras condi??es cl?nicas avaliadas, para todos os marcadores, sugerindo uma poss?vel associa??o destes marcadores com a progress?o desta doen?a, onde quanto maior a perda de suporte periodontal, maior a quantidade do infiltrado inflamat?rio e maior n?mero de c?lulas imunomarcadas. / Periodontal disease is a chronic inflammatory condition primarily caused by bacteria in dental biofilm, which interact with the host, thus determining the nature of the resulting disease. Despite the wide knowledge about the pathogenesis of these diseases, the exact composition of the T cell profile during the active phase of the disease (Th1, Th2 or Th17) remains unknown. This study aimed to evaluate by immunohistochemical expression, the presence of the markers (IL-17, IL-23 and ROR?t), involved in Th17 response in clinically healthy gingiva cases (n = 32), biofilm-induced gingivitis (n = 30), chronic periodontitis (n = 32) and aggressive periodontitis (n = 25), in order to analyze if the expression and/or distribution of these molecules in lymphocytes and macrophages, present in the inflammatory infiltrate of periodontal tissue, influences the tissue destruction observed in these diseases. The morphological analysis of cases was performed which assessed the intensity of the inflammatory infiltrate in mild, moderate and intense. For each case, in the area with the most representative immunostaining, 5 fields were chosen and analyzed, both for the intensity of the inflammatory infiltrate as for the quantity of immunostained cells, based on predetermined scores: score 0 (absence of inflammatory infiltrate/immunostaining), score 1 (the infiltrate/immunostaining covered less than 25% of the field area), score 2 (the infiltrate/immunostaining occupied between 25 and 50%) and score 3 (infiltrate/immunostaining present in over 50% of the field area). From this, a median was generated representing each case. The intensity of the inflammatory infiltrate correlated with the disease progression, in other words, it was crescent from clinically healthy gingiva to aggressive periodontitis (P <0.001). It was detected the presence of IL-17, IL-23 and ROR?t in most of the evaluated cases and the number of immunostained cells correlated with the intensity of the inflammatory infiltrate (P <0.001) and with the clinical parameters analyzed (P <0.001), showing a positive correlation, mainly moderate. Aggressive periodontitis showed a higher percentage of immunostaining for all markers in relation to other clinical conditions assessed, suggesting a possible association of these markers with the progression of this disease, in which the higher the loss of periodontal support, the greater the amount of inflammatory infiltrate and larger the number of immunostained cells.

Made available in DSpace on 2014-12-17T15:31:01Z (GMT). No. of bitstreams: 1 AlexandrePM.pdf: 292749 bytes, checksum: d4140e747a62ba0e126ab6b205344290 (MD5) Previous issue date: 2007-02-15 / The relationship between periodontal and cardiovascular diseases is a reality in the current days. The knowledge about the role of periodontal disease as a risk factor to cardiovascular disease from dentistry and physicians is very important to try to eliminate this risk factor. The aim of this work was to investigate, using a questionary, if physicians and dentistry are aware about this relationship and if they think that it is important. These forms were distributed in 4 groups: cardiologists (n=90), physicians from other specialist (POS) (n=110), periodontists (n=35) and dentistry from other specialist (DOE) (n=85). We had a loss off 32,4% of the total sample due to a lack of response of some professionals. Our results showed that all cardiologists, all dentists and 68,2% of POE said that they had gotten information about the relationship between periodontal and cardiovascular disease, and just 6 POS don t believe that this relationship can occur. When questioned if, even when a periodontal disease is diagnosed, there is a habit to treat or orient the patient for treatment, 29,5% of cardiologists answered no, 25,5% of POE also answered that they did not orient and only 1 DOE answered that he did not treat nor orient. All periodontists said that treat their patients when periodontal disease was identify. The physicians seem not to find important the relationship that exist between periodontal and cardiovascular diseases and the dentistry have shown a good knowledge about this subject. Maybe, if physicians and dentistry work together, the incidence of cardiovascular disease decreases / A poss?vel rela??o entre a doen?a periodontal e a cardiovascular ? uma realidade nos dias atuais. O conhecimento sobre o papel da doen?a periodontal como fator de risco para doen?as cardiovasculares por cirurgi?es-dentistas e m?dicos ? muito importante na tentativa de eliminar esse fator de risco. O objetivo deste trabalho foi de investigar, atrav?s de um question?rio, se m?dicos e dentistas t?m conhecimento sobre tal rela??o e se consideram importante. Os question?rios foram distribu?dos em 4 grupos: cardiologistas (n=90), m?dicos de outras especialidades (MOE) (n=110), periodontistas (n=35) e cirurgi?es-dentistas de outras especialidades (CDOE) (n=85). Tivemos uma perda de 32,4% da amostra total devido a uma n?o resposta de alguns profissionais. Nossos resultados mostraram que todos os cardiologistas, todos os CDs e 68,2% dos MOE disseram ter obtido informa??o sobre a rela??o entre a doen?a periodontal e cardiovascular, e apenas 6 MOE n?o acreditam que essa rela??o possa existir. Quando questionados se, mesmo ao ser diagnosticada a doen?a periodontal, existia o h?bito de tratar ou encaminhar os pacientes para tratamento, 29,5% dos cardiologistas responderam que n?o, 25,5% dos MOE tamb?m disseram n?o encaminhar e apenas 1 CDOE respondeu n?o tratar nem encaminhar. Todos os periodontistas disseram tratar os pacientes com doen?as periodontais quando identificadas. Os m?dicos parecem n?o creditar import?ncia na rela??o que existe entre a doen?a periodontal e a cardiovascular e os CDs mostraram um bom conhecimento sobre a rela??o. Talvez, se m?dicos e CDs trabalharem juntos, a incid?ncia da doen?a cardiovascular decres?a

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2017-02-21T22:16:47Z No. of bitstreams: 1 Disserta?ao L?lia Paula S Santos.pdf: 2382410 bytes, checksum: 0aad3a2e285b3ca2ee6f0efdce051973 (MD5) / Made available in DSpace on 2017-02-21T22:16:47Z (GMT). No. of bitstreams: 1 Disserta?ao L?lia Paula S Santos.pdf: 2382410 bytes, checksum: 0aad3a2e285b3ca2ee6f0efdce051973 (MD5) Previous issue date: 2016-03-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Oral health problems are increasingly receiving recognition as important factors that impact quality of life. Socio-dental survey instruments can be used to evaluate this impact, and along with clinical evaluations, are important sources of knowledge for the planning and implementation of health policies that allow the intervention positively. OBJECTIVE: Evaluate the association between oral clinical condition and the perception of its impact on quality of life, taking into account the physical, psychological and social dimensions related to quality of life of individuals attended to in the public health units of Feira de Santana - BA. METHODS: Cross-sectional study developed with adult individuals attended in Family Health Units and the Center for Diabetic and Hypertensive care in the municipality of Feira de Santana--BA. The data were collected through the interviews with application of a questionaire with issues about socioeconomic, demographic, lifestyle, general and oral health condition characteristics, conducting a full oral clinical examination and application of OHIP-14. The statistical analysis included descriptive evaluation of the variables of interest and comparing the total OHIP scores according to the variables of oral health. Association measurements, prevalence ratio (PR) and 95% confidence interval (95% CI), between the occurrence of periodontitis and / or presence of dental caries and the impact on quality of life were obtained by poisson regression with robust variance. RESULTS: they were presented as two scientific articles. In the first article, the findings showed statistically significant associations between dental caries (PRadjusted: 1.43; 95% CI: 1.02-2.01) and severe periodontitis (PRadjusted: 1.72; 95% CI: 1.00-2.98) with the impact of oral health on quality of life. In the second article, it was observed the association between the presence of dental caries combined with periodontitis and the impact of quality of life in oral health (PRadjusted: 1.63; 95% CI: 1.03-2.59). CONCLUSIONS: The findings of this study showed that precarious oral health and accumulation of dental needs (dental caries and periodontitis combined) are associated with self-perceived oral health according the oral health impact profile (OHIP). / Problemas de sa?de bucal t?m sido cada vez mais reconhecidos como fatores importantes que causam impacto na qualidade de vida. Instrumentos sociodentais s?o utilizados para obten??o deste impacto e juntamente com a avalia??o cl?nica s?o importantes fontes de conhecimento para o planejamento e a implementa??o de pol?ticas p?blicas de sa?de que possibilitem a interven??o de forma positiva. OBJETIVO: avaliar a associa??o entre condi??o cl?nica bucal e a percep??o do seu impacto na qualidade de vida de indiv?duos atendidos na rede p?blica de Feira de Santana ? BA.M?TODO: Estudo transversal desenvolvido com indiv?duos adultos atendidos em Unidades de Sa?de da Fam?lia e no Centro de Atendimento ao Diab?tico e Hipertenso do munic?pio de Feira de Santana ? BA. Os dados foram coletados por meio de entrevista com aplica??o de question?rio que abordava itens sobre caracter?sticas socioecon?mico-demogr?ficas, estilo de vida, condi??o de sa?de geral e bucal, realiza??o de exame cl?nico bucal completo e aplica??o do OHIP-14. A an?lise estat?stica compreendeu avalia??o descritiva das vari?veis de interesse e compara??o dos escores totais do OHIP segundo as vari?veis da condi??o bucal. Medidas de associa??o, raz?o de preval?ncia (RP) e intervalo de confian?a a 95% (IC95%), entre ocorr?ncia da periodontite e/ou presen?a de c?rie dent?ria e o seu impacto na qualidade de vida foram obtidas por meio da Regress?o de Poisson com vari?ncia robusta.RESULTADOS: Foram apresentados em forma de dois artigos cient?ficos. No primeiro artigo, os achados mostraram associa??es estatisticamente significantes entre a ocorr?ncia de c?rie dent?ria (RPajustada: 1,43; IC95%: 1,02-2,01) e da periodontite grave (RPajustada: 1,72; IC95%: 1,00-2,98) com o impacto da sa?de bucal na qualidade de vida. No segundo artigo, foi observada a associa??o entre presen?a de c?rie dent?ria combinada a periodontite com o impacto da qualidade de vida na sa?de bucal (RPajustada: 1,63; IC95%: 1,03-2,59). CONCLUS?ES: Os achados deste estudo mostraram que a condi??o bucal prec?ria e ac?mulo de necessidades bucais (c?rie dent?ria e periodontite combinadas) est?o associados coma autopercep??o da sa?de bucal conforme o perfil de impacto na sa?de bucal(OHIP).

Made available in DSpace on 2015-03-03T15:38:42Z (GMT). No. of bitstreams: 1 BrunaRMS_TESE.pdf: 1789056 bytes, checksum: 3971348cc480ce2ec7b8b377a649483e (MD5) Previous issue date: 2011-02-17 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Periodontal disease is an inflammatory condition of infectious nature characterized by destruction of protecting and supporting dental tissues. It happens as a response produced by the host when attacked by microorganisms. Several factors are involved in this process. Among them, cytokines are key regulatory molecules in this immune response, playing a role either protective and/or destructive in lesion progression. Thus, this study investigated the immunohistochemical expression of IFN- , GATA-3, IL-17, IL-23, IL-6 and TGF- in gingival tissues of humans, in an attempt to gain a better understanding of the participation of Th1, Th2 and Th17 immune responses in the development of periodontal disease processes. To this end, eighty-two samples of gingival tissues were divided into three groups: Group 1 = 15 (samples of healthy gum tissue as controls), Group 2 = 36 (samples with chronic gingivitis) and Group 3 = 31 (samples with chronic periodontitis). All cases were submitted to morphological analysis from sections stained with hematoxylin and eosin and then subjected to staining by immunohistochemistry using the streptavidin-biotin method. Results showed positive labeling for all proteins. Nonetheless, we observed a greater expression of Th1 cytokines and Th17 cells in group 3. We found statistically significant difference between TGF- expression and the clinical condition of the samples (p=0.02). We conclude that Th1 and Th17 responses may act synergistically in the destructive process of periodontal tissue, overlapping the Th2 response that was also present in these tissues / A doen?a periodontal ? uma condi??o inflamat?ria de car?ter infeccioso, caracterizada pela destrui??o dos tecidos de prote??o e sustenta??o dent?rios, face ? resposta produzida pelo hospedeiro frente ?s agress?es sofridas pelos microrganismos. V?rios fatores est?o envolvidos nesse processo, sendo as citocinas as principais mol?culas reguladoras dessa resposta imune, desempenhando um papel protetor e/ou destrutivo na progress?o da les?o. Diante disso, este experimento investigou a express?o imuno-histoqu?mica de IFN- , GATA-3, IL-17, IL-23, IL-6 e TGF- em tecidos gengivais de humanos, na tentativa de se obter um maior entendimento da participa??o das respostas imunes Th1, Th2 e Th17 no desenvolvimento destes processos patol?gicos. Para tanto, oitenta e duas amostras de tecidos gengivais foram subdivididas em tr?s grupos: Grupo 1=15 (amostras de tecido gengival saud?vel-controle), Grupo 2=36 (amostras com gengivite cr?nica) e Grupo 3=31 (amostras com periodontite cr?nica). Todos os casos foram submetidos ? an?lise morfol?gica a partir de cortes corados em hematoxilina e eosina e, posteriormente, submetidas ? t?cnica de colora??o pela imuno-histoqu?mica atrav?s do m?todo da Estreptoavidina-Biotina. Os resultados mostraram positividade de marca??o para todas as prote?nas, sendo observada uma maior tend?ncia de marca??o para as citocinas das respostas Th1 e Th17 no grupo 3. Diferen?a estatisticamente significativa foi verificada entre a express?o de TGF- e a condi??o cl?nica das amostras (p=0,02). Assim, podemos concluir que as respostas Th1 e Th17 podem atuar sinergicamente no processo destrutivo dos tecidos periodontais, sobrepondo-se ? resposta Th2 que tamb?m se encontrou presente nestes tecidos

Made available in DSpace on 2014-12-17T15:30:49Z (GMT). No. of bitstreams: 1 MarianneNMN_capa_ate_secao3.pdf: 245783 bytes, checksum: 4f928aea72870694ac1bb2867b0c9fcb (MD5) Previous issue date: 2007-11-01 / The presence of fixed orthodontics appliances interfere on sanitation, allowing periodontal diseases to appear, despite the fact patients keep on visiting the dentist every month. This research aims to determine a protocol for the mechanical control of the dental biofilm performed by the professional. A protocol that was able to maintain the periodontal health of the patients under orthodontic treatment with fixed appliances, and in order to do so, it used a non-controlled, randomized and blind clinical essay. The sample involved 40 adolescents who were under the installation of fixed orthodontics appliances and it was divided in three groups, as follows: monthly controlled group (group 1) composed of 11 patients, the quarterly controlled group (group 2) with 16 patients and the semestrial controlled group (group 3) with 13 patients. For data collection, an interview and clinical exams with probing depth measurement, quantity of keratinized mucosa, Gingival Index and the Plaque Index were used. On the initial exam all patients received brushing guidelines as well as the professional control of dental biofilm, with periodontal scaler, Robinson s brush and prophylactic paste. However, Group 1 returned every month for control procedures; Group 2 every three months and Group 3 after six months. The intervention had a six-month duration (for all the three groups), when all the exams were repeated by another examiner who did not know which group each patient was inserted in. Finally, the research concluded that despite the fact there is no statistically significant difference among the three groups, clinically the patients from the monthly group presented a better response to professional control, with less accumulation of dental biofilm and less rate of gingival inflammation. Thus, the mechanical control of the dental biofilm performed by the professional could not avoid gingival increase, characterized by the raise of probing depth measurement, neither the quantity of keratinized mucosa / A presen?a do aparelho ortod?ntico fixo dificulta a higieniza??o, tornando comum o aparecimento de problemas periodontais nestes pacientes, apesar de manterem contato todo m?s com o dentista. O objetivo desta pesquisa foi determinar um protocolo, de controle mec?nico do biofilme dent?rio executado pelo profissional, que fosse capaz de manter a sa?de periodontal de pacientes em tratamento ortod?ntico com aparelho fixo, para tanto se empregou um ensaio cl?nico, n?o-controlado, randomizado e cego. A amostra envolveu 40 adolescentes na fase de instala??o do aparelho ortod?ntico fixo, e foi dividida em tr?s grupos sendo eles: o grupo do controle mensal (grupo 1) composto de 11 pacientes, o grupo do controle trimestral (grupo 2) com 16 pacientes e o grupo do controle semestral (grupo 3) com 13 pacientes. Para a coleta de dados adotou-se uma entrevista e exame cl?nico com medi??es da profundidade de sondagem, quantidade de mucosa ceratinizada, ?ndice Gengival e o ?ndice de Placa. No exame inicial todos os pacientes receberam orienta??o de escova??o e controle profissional de biofilme dent?rio, com curetas periodontais, escova de Robinson e pasta profil?tica. Sendo que o grupo 1 retornou a cada m?s para o controle; o 2 a cada tr?s meses e o 3 ap?s seis meses. A interven??o teve uma dura??o de seis meses (para os tr?s grupos), quando todos os exames foram repetidos por outro examinador que desconhecia a qual grupo pertencia cada paciente. Ao final da pesquisa foi poss?vel concluir que apesar de n?o existir diferen?a estatisticamente significativa entre os tr?s grupos, clinicamente os pacientes do grupo mensal apresentaram uma melhor resposta ao controle profissional, com menor ac?mulo de biofilme dent?rio e menor grau de inflama??o gengival. Contudo, o controle mec?nico do biofilme dent?rio executado pelo profissional n?o conseguiu prevenir o aumento gengival, caracterizado pela eleva??o da profundidade de sondagem, bem como da quantidade de mucosa ceratinizada

Made available in DSpace on 2014-12-17T15:31:01Z (GMT). No. of bitstreams: 1 BrunaAA.pdf: 501159 bytes, checksum: 30bc6bac9bef1fd1e36f669902ea0e7c (MD5) Previous issue date: 2007-02-27 / The literature has shown a relation between periodontics and the removable partial denture (RPD), with progressive destruction observed in the support structures. The aim of this study was to clinically assess periodontal condition in users of removal partial denture (RPD), and compare right abutments teeth, indirect abutments and controls before installation and after 1 year, in addition to comparing tooth-supported and tooth mucosa-supported abutments. A total of 50 patients, 32 women and 18 men, mean age of 45 years, took part in the study. The patients were examined by a single examiner at prosthesis installation and after 3, 6, 9 and 12 months. The following were verified at each examination: Probe Depth (PD), Plaque Index (PI), Gingival Index (GI), the amount of Keratinized Mucosa (KM), Gingival Recession (GR) and Dental Mobility (DM); in addition patients received oral hygiene orientation, accompanied by prophylaxis, periodontal scaling and root planing (PSRP), when necessary. Analysis of Variance (ANOVA) with Tukey-Kramer post test was used to assess the dependent variables (PD, PI, KM, GR) of the three groups over time while Friedman s test was used for GI. To assess the outcomes of prosthesis type in the right abutment group, a confidence interval-based analysis was performed. The results showed that the control group was the least compromised in all the variables studied. With respect to development of the groups over time, it was verified that the measures for GR, PD, GI and KM increased from initial examination to 1 year of use in all the groups, but only PI showed a significant increase. There was a non-discriminatory low prevalence of dental mobility. The tooth mucosa-supported prosthesis had significantly higher values for GR, GI and PI and significantly lower ones for KM when compared to tooth-supported. Over time, both types of prostheses showed no significant differences from initial to final examination for the variables GR, PD, KM and GI, with PI significant only for tooth-supported. The results showed that the teeth most involved in RPD design had greater potential of periodontal damage, probably because of greater dental biofilm accumulation. Abutments elements adjacent to the free extremities had less favorable periodontal condition than those adjacent to interpolated spaces, but the use of RPD did not worsen the initial condition / A literatura tem mostrado uma rela??o entre a periodontia e a pr?tese parcial remov?vel (PPR), observando-se destrui??es progressivas das estruturas de suporte. O prop?sito do presente trabalho ? avaliar clinicamente a condi??o periodontal em usu?rios de pr?tese parcial remov?vel (PPR), comparando os dentes pilares diretos, pilares indiretos e os dentes n?o envolvidos no desenho da pr?tese, antes da instala??o e ap?s 1 ano de uso. E ainda comparar os pilares diretos de PPR dento-suportada e dento-muco-suportada. Um total de 50 pacientes, 32 do sexo feminino e 18 do masculino, com idade m?dia de 45 anos participaram da pesquisa. Os pacientes foram examinados no momento da instala??o da pr?tese e ap?s 3, 6, 9 e 12 meses, por um ?nico examinador. Em cada exame foram verificados: a recess?o gengival (RG) profundidade de sondagem (PS), o ?ndice de placa (IP), o ?ndice gengival (IG), a quantidade de mucosa ceratinizada (MC), e a mobilidade dent?ria (MB), al?m da orienta??o de higiene oral, acompanhada de profilaxia e raspagem e alisamento corono - radicular (RACR) quando necess?ria. Para avaliar o comportamento das vari?veis dependentes (RG, PS, IP, MC) dos tr?s grupos ao longo do tempo, o teste de escolha foi a an?lise de vari?ncia de tend?ncia linear com o p?s teste de Tukey-Kramer, e para a vari?vel IG foi realizado o teste de Friedman. Para avaliar os desfechos com rela??o ao tipo de pr?tese no grupo pilar direto, foi feita an?lise a partir do intervalo de confian?a. Os resultados mostraram que o grupo controle foi o que apresentou menor comprometimento em todas as vari?veis estudadas. Com rela??o ao desenvolvimento dos grupos no decorrer do tempo, verificou-se que as m?dias da RG, PS, IG e MC aumentaram do exame inicial para 1 ano de uso, em todos os grupos, mas somente o IP mostrou aumento significativo. A mobilidade dent?ria foi pouco discriminat?ria por apresentar baixa preval?ncia. No que diz respeito ? diferen?a entre as pr?teses, a pr?tese dento-muco-suportada mostrou valores significativamente superiores de RG, IG e IP e significativamente inferiores MC em rela??o ? dento-suportada. Ao longo do tempo, os dois tipos de pr?tese n?o apresentaram diferen?as significativas do exame inicial para o final para as vari?veis: RG, PS, MC e IG, j? IP foi significativo somente na pr?tese dento-suportada. Os resultados obtidos mostraram que os dentes mais envolvidos no desenho da PPR apresentaram maior potencial de destrui??o periodontal, provavelmente por apresentarem maior ac?mulo de biofilme dent?rio. Elementos pilares adjacentes a extremidades livres apresentaram condi??o periodontal menos favor?vel do que os pilares adjacentes a espa?os intercalados, por?m o uso de PPR n?o agravou a condi??o inicial

Made available in DSpace on 2014-12-17T15:32:20Z (GMT). No. of bitstreams: 1 RoseaneCV_DISSERT.pdf: 1364911 bytes, checksum: 1503d99f23893ef62ce15add6f41141c (MD5) Previous issue date: 2012-02-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Periodontal disease is a complex inflammatory and infectious condition that immune host, front of the microbial aggressions, can lead to disease progression, resulting in tissue destruction. The tissue damage induces the release of regulatory molecules, which protective roles and / or destructive, including proteins VEGF (vascular endothelial growth factor vascular) and HIF-1 ? (hypoxia-induced factor ? -1). Thus, this study investigated, quantitatively and comparatively, the immunohistochemical expression of VEGF (vascular endothelial growth factor) and HIF-1 ? (hypoxia induced factor 1-?), proteins involved in inflammation, angiogenesis and hypoxia, in human gingival tissues. Therefore, 75 samples of gingival tissues were examined. Thirty samples were chronic periodontitis, 30 with chronic gingivitis and 15 healthy gingival. After sections analysis, positives and negatives inflammatory and endothelial cells in the connective tissue were counted and converted into percentage. Data were statistically analyzed using Kruskal-Wallis test and Spearman correlation. The results showed that both proteins marked. It was observed higher immunoreactivity for HIF-1 ? at chronic gingivitis and periodontitis specimens compared to healthy sites, however, no statistically significant differences were observed among them (p>0.05). The VEGF immunostaining showed similarity among the cases of periodontitis, gingivitis and healthy gingiva. Moderate and positive correlation statistically significant differences were verified for the expressions of VEGF and HIF-1? in gingival health (r = 0,529, p = 0.04). Thus, it can be conclude that possibly the route of HIF-1 ?, is activated in periodontal disease may have involvement of the protein in pathogenesis and progression of disease, and that activation of VEGF, can be in addition to being triggered transcription by HIF-1 ? may be also influenced by other additional factors such as the action of periodontal microorganisms endotoxins / A doen?a periodontal ? uma condi??o infecciosa e inflamat?ria complexa em que a resposta imune do hospedeiro, frente aos produtos microbianos, pode conduzir ? progress?o da doen?a. A agress?o tecidual induz a libera??o de mol?culas reguladoras, que desempenham pap?is protetores e/ou destrutivos, incluindo as prote?nas VEGF (Fator de crescimento endotelial vascular) e o HIF-1 ? (Fator induzido por hip?xia -1 ?). Diante disso, este estudo buscou analisar, de forma quantitativa e comparativa, a express?o imuno-histoqu?mica destas prote?nas, envolvidas nos processos de angiog?nese e hip?xia, observadas em condi??es inflamat?rias. Para tanto, 75 amostras de tecidos gengivais foram examinadas. Destas, 30 foram de periodontite cr?nica, 30 de gengivite cr?nica e 15 de gengivais saud?veis. Foram contadas, as c?lulas inflamat?rias e endoteliais, positivas e negativas, no tecido conjuntivo fibroso, e posteriormente, convertidas em porcentagem. Os dados foram analisados estatisticamente por meio do teste de Kruskal-Wallis e Correla??o de Spearman. Os resultados mostraram imunoexpress?o para ambas as prote?nas. Foi observada, uma elevada imunoexpress?o para HIF-1 ?, nos esp?cimes de periodontite e gengivites cr?nicas, em rela??o aos s?tios saud?veis, por?m, sem diferen?as estat?sticas entre elas (p>0,05). A imunoexpress?o do VEGF demonstrou similaridade, entre os casos de periodontite, gengivite e gengiva saud?vel. Correla??o positiva moderada e diferen?a estatisticamente significativa foram verificadas para as express?es do VEGF e HIF-1?, em gengivais saud?veis (r=0.529; p= 0.04). Desta forma, pode-se concluir que, possivelmente a via de ativa??o do HIF-1 ?, est? ativada na doen?a periodontal, podendo haver participa??o desta prote?na, na patog?nese e progress?o da doen?a periodontal e que a ativa??o do VEGF, al?m de ser desencadeada via transcri??o do HIF-1 ?, pode ser tamb?m, influenciada por outros fatores adicionais, como, por exemplo, a??o das endotoxinas bacterianas periodontopatog?nicas