Yes / The proteins of SMC family are characterised by having Walker A and B sites. The Escherichia coli RecN protein is a prokaryotic member of SMC family that involved in the induced excision of Tn10 and the repair of the DNA double strand breaks. In this work, the Walker A nucleotide binding site of the E. coli RecN protein was mutated by changing the highly conserved lysine residue 35 to the aspartic acid (D), designated as recN(K35D). Reverse genetics was utilized to delete the entire recN gene (Delta recN108) or introduce the recN(K35D) gene into the E. coli chromosomal DNA. The recN(K35D) cells showed decreasing in the frequency of excision of Tn10 from gal76
Identifer | oai:union.ndltd.org:BRADFORD/oai:bradscholars.brad.ac.uk:10454/10558 |
Date | 06 February 2011 |
Creators | Youssef, M.M., Al-Omair, M.A., Picksley, Stephen M. |
Source Sets | Bradford Scholars |
Language | English |
Detected Language | English |
Type | Article, Published version |
Rights | (c) 2014 Elsevier. This is an Open Access article distributed under the Creative Commons BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/3.0/), CC-BY-NC-ND |
Page generated in 0.0024 seconds