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Previous issue date: 2013-07-19 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Perciformes are dominant in the marine environment, characterized as the largest and most diverse fish group. Some families, as Gerreidae, popularly known as silver jennies, carapebas, or mojarras have a high economic potential to marine fish farming, natural explotation and game fishing. Genetic information of these species are of fundamental importance for their management and production. Despite exist over 13,000 marine fish species described, only 2% were cytogenetically analyzed and less than 1% have some reproductive characteristics known. Induced breeding, cytogenetic characterization and cryopreservation of gametes, represent important areas in applied fish studies. In this project cytogenetic analyzes were performed to acess genetic aspects of Gerreidae species, distributed in coastal and estuarine regions of Northeast Brazil. Different methods for identifying chromosomal regions were employed using conventional techniques (Ag-NORs, C-banding), staining with base-specific fluorochromes (DAPI-CMA3), and physical mapping of ribosomal genes 18S and 5S rDNA, through hybridization in situ with fluorescent probes (FISH). The six species analyzed showed remarkable chromosome conservatism. The 18S and 5S ribosomal genes when analyzed in phylogenetic perspective demonstrate varied evolutionary dynamics, suggesting ocurrence of stasis process in some groups and greater dynamism in others. Double FISH with 18S and 5S probes showed both how efficient cytotaxonomic markers in the homogeneous karyotypes of this group of species. The karyotypic pattern identified in addition to the evolutionary aspects of karyotype, are suggestive of existence of low potential of post-zygotic barrier, prompting further research to prospect for artificial interspecific hybridization of these species of commercial importance / Os Perciformes s?o dominantes no ambiente marinho, contituindo a maior e mais diversificada ordem de peixes dentre os tele?steos. Muitas de suas fam?lias, como os Gerreidae, conhecidos popularmente como carapicus, carapebas, ou mojarras, t?m um alto potencial econ?mico, no que se diz respeito ? piscicultura marinha, extrativismo e pesca esportiva. Informa??es gen?ticas destas esp?cies s?o de fundamental import?ncia para seu manejo e produ??o. Mesmo assim, das 13.000 esp?cies de peixes marinhos descritos, apenas 2% foram estudadas sob o ponto de vista citogen?tico e menos de 1% sobre suas caracter?sticas reprodutivas. A reprodu??o induzida, a citogen?tica e a criopreserva??o de gametas, representam importantes ?reas aplicadas de estudo em peixes. No presente trabalho an?lises citogen?ticas foram empregadas na caracteriza??o gen?tica de esp?cies da fam?lia Gerreidae, ocorrentes no litoral do Nordeste do Brasil. Diferentes m?todos de identifica??o de regi?es cromoss?micas foram empregados por meio de t?cnicas convencionais (Ag-RONs, bandamento C), colora??o com fluorocromos base-espec?ficos (DAPI-CMA3), e mapeamento cromoss?mico de genes ribossomais marcadores DNAr 18S e 5S, atrav?s da hibrida??o in situ com sondas fluorescentes (FISH). As seis esp?cies analisadas revelaram marcante conservadorismo cromoss?mico. Os genes ribossomais 18S e 5S quando analisados em perspectiva filogen?tica, demonstram din?mica evolutiva variada, podendo apresentar estase em alguns grupos e maior dinamismo em outros. As an?lises por duplo-FISH dos s?tios 18S e 5S se revelaram eficientes marcadores citotaxon?micos nos cari?tipos homog?neos deste grupo de esp?cies. Os padr?es cariot?picos identificado, al?m dos aspectos evolutivos do cari?tipo identificados, s?o sugestivos de baixo potencial de barreiras p?s-zig?ticas, instigando pesquisas futuras de prospec??o de hibrida??o interespec?fica destas esp?cies de valor comercial
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/12653 |
| Date | 19 July 2013 |
| Creators | Calado, Leonardo Luiz |
| Contributors | CPF:56615043491, http://lattes.cnpq.br/8437464961129518, Yasui, George Shigueki, CPF:28153460870, http://lattes.cnpq.br/9501210162294651, Garcia J?nior, Jos?, CPF:27234944893, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4739386P6, Souza, Liliane de Lima Gurgel, CPF:03095950454, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4701211U3, N?brega, Marcelo Francisco de, CPF:14839619824, http://lattes.cnpq.br/6194914254799842, Molina, Wagner Franco |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Biotecnologia, UFRN, BR, Biotecnologia Industrial; Biotecnologia em Agropecu?ria; Biotecnologia em Recursos Naturais; Biotecn |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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