Brain-derived neurotrophic factor (BDNF), an important neural growth factor, has gained
growing interest in neuroscience, but many influencing physiological and analytical aspects still
remain unclear. In this study we assessed the impact of storage time at room temperature, repeated
freeze/thaw cycles, and storage at 80 C up to 6 months on serum and ethylenediaminetetraacetic
acid (EDTA)-plasma BDNF. Furthermore, we assessed correlations of serum and plasma BDNF
concentrations in two independent sets of samples. Coefficients of variations (CVs) for serum BDNF
concentrations were significantly lower than CVs of plasma concentrations (n = 245, p = 0.006).
Mean serum and plasma concentrations at all analyzed time points remained within the acceptable
change limit of the inter-assay precision as declared by the manufacturer. Serum and plasma BDNF
concentrations correlated positively in both sets of samples and at all analyzed time points of the
stability assessment (r = 0.455 to rs = 0.596; p < 0.004). In summary, when considering the acceptable
change limit, BDNF was stable in serum and in EDTA-plasma up to 6 months. Due to a higher
reliability, we suggest favoring serum over EDTA-plasma for future experiments assessing peripheral
BDNF concentrations.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:89563 |
| Date | 07 February 2024 |
| Creators | Polyakova, Maryna, Schlögl, Haiko, Sacher, Julia, Schmidt-Kassow, Maren, Kaiser, Jochen, Stumvoll, Michael, Kratzsch, Jürgen, Schröter, Matthias L. |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 1189 |
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