The effect of salinity on phosphorus (P) absorption kinetics by 'Russet Burbank' cv. potato propagated by tissue culture technique was studied in the laboratory. Nine clones of the cultivar were evaluated for their salt tolerance at the vegetative stage by using in vitro culture. Four concentrations of NaCl (0, 40, 80, and 120 mM) were used in the experiments. The first experiment was carried out to study the effect of salinity on P absorption by using radiophosphorus ($ sp{32}$P) as a marker. P absorption showed a quadratic relationship with time. In general, there was a significant reduction in P absorption as the NaCl concentration increased in the nutrient medium. However, the root tip concentration of $ sp{32}$P increased with increased salt concentration. When P concentration in the nutrient medium was increased, $ sp{32}$P activity was also increased with time at 120 mM salt concentration. The activity of radiophosphorus decreased on root tips when P concentration in the nutrient medium was increased to 2.5 mM P. The second experiment was conducted in tissue culture using nodal cutting bioassay. The plant morphological parameters of shoot length, shoot fresh weight, shoot dry weight, root length, root fresh weight, and root dry weight were reduced for all nine clones as salinity increased. The clone PEI#1 (tu) exhibited high salt tolerance characteristics.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.27352 |
| Date | January 1997 |
| Creators | Kalifa, Ali. |
| Contributors | Barthakur, N. N. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Natural Resource Sciences.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001576705, proquestno: MQ29727, Theses scanned by UMI/ProQuest. |
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