CoordenaÃÃo de AperfeiÃoamento de NÃvel Superior / A Acupuntura (Ac) e a Eletroacupuntura (EAc) tem sido amplamente utilizadas na prÃtica clÃnica para o tratamento de vÃrias doenÃas. A Medicina Tradicional Chinesa (MTC) preconiza o uso dos acupontos Zusanli (E-36) e Zhongwan (RM-12) para alÃvio dos sintomas de diabetes mellitus. Por aÃÃo da Ac e EAc, ocorre liberaÃÃo de opiÃides endÃgenos,
incluindo β-endorfinas, que comprovadamente estimulam a secreÃÃo de insulina. O objetivo desse estudo foi avaliar os efeitos da Ac e EAc nos acupontos E-36 e RM-12 sobre as
concentraÃÃes de glicose e lactato no sangue, fÃgado e rim de ratos sadios. No presente estudo, 48 ratos foram divididos em 4 grupos (Valores Basais, Ac, EAc10Hz e EAc100Hz) e
subdivididos em 2 subgrupos de acordo com os tempos de coleta dos substratos (T-30: imediatamente apÃs o tÃrmino da sessÃo de Ac ou EAc e T-60: 30 minutos apÃs o tÃrmino
da sessÃo). Todos os ratos foram anestesiados e os grupos experimentais (Ac, EAc10Hz e EAc100Hz) foram submetidos, respectivamente, à Ac e à EAc (10 Hz e 100 Hz) nos
acupontos E-36 e RM-12, durante 30 minutos. Foram dosadas as concentraÃÃes de glicose, lactato e LDH no sangue, fÃgado e rim dos animais nos tempos T-30 e T-60. A estimulaÃÃo
dos acupontos E-36 e RM-12 por EAc de 10Hz reduziu significativamente a glicemia em ratos sadios, ao tÃrmino da estimulaÃÃo elÃtrica (190,80Â24,88 vs. 138,50Â21,09, p>0,001) e 30 minutos mais tarde (186,30Â23,76 vs. 134,10Â25,65, p>0,001). Utilizando-se uma freqÃÃncia 10 vezes maior (100Hz) demonstrou-se resultado semelhante (T-30, 186,30Â23,76 vs. 99,17Â8,79, p>0,001; e T-60, 190,80Â24,88 vs. 113,40Â7,37, p>0,001). A Ac nÃo alterou significativamente a glicemia. Houve tambÃm reduÃÃo significante das concentraÃÃes de LDH imediatamente apÃs o tÃrmino da sessÃo de acupuntura e 30 minutos depois, tanto nos animais submetidos à AC (T-30, 518,20 43,31 vs. 354,80Â44,66, p<0,001; T-60, 637,80Â61,01 vs. 325,70Â10,54, p<0,001) quanto à EA (10Hz: T-30, 518,20Â
43,31 vs. 286,50Â59,86, p<0,001; T-60, 637,80Â61,01 vs 268,80Â43,51, p<0,001 e 100Hz: T-30, 518,20Â 43,31 vs. 213,80Â24,85, p<0,001; T-60, 637,80Â61,01 vs 188,20Â24,00,
p<0,001), sugerindo um menor metabolismo anaerÃbio. No presente estudo, a reduÃÃo significante do lactato sÃrico 30 minutos apÃs o inÃcio do estudo em ratos submetidos à EAc
com 100 Hz sugere prevalÃncia da glicÃlise aerÃbica (2,10Â0,23 vs. 1,19Â1,02, p<0,001). Resultado semelhante foi observado nos nÃveis de lactato no fÃgado (T-60, 10 Hz: 1,92Â0,42 vs. 0,69Â0,29, p<0,001 e 100 Hz: 1,92Â0,42 vs. 0,44Â0,21, p<0,001) e rim (T-30, 100 Hz:
1,86Â0,87 vs. 0,91Â0,38, p<0,05). Os resultados deste estudo mostram que a Ac e a EAc nos animais hiperglicÃmicos podem reduzir as concentraÃÃes de glicose no sangue.
FreqÃÃncias maiores tem maior efeito hipoglicemiante. A reduÃÃo da glicemia deve ser conseqÃÃncia da aÃÃo de opiÃides endÃgenos e da β-endorfina, como sugerido em estudos anteriores. O mecanismo e reduÃÃo das concentraÃÃes de lactato e LDH parece estar
relacionado a uma prevalÃncia da glicÃlise aerÃbica. / Acupuncture (Ac) and electroacupuncture (EAc) have been widely used in clinical practice
for the treatment of various diseases. Traditional Chinese medicine (TCM) recommends the use of acupoints Zusanli (E-36) and Zhongwan (RM-12) for relief of symptoms of diabetes mellitus. Ac and EAc stimulation may release some endogenous opioids, including - endorphins, enhancing insulin secretion. The aim of this study was to evaluate the effects of Ac and EAc on acupoints ST-36 and RM-12 on glycemia, lactacemia and liver and kidneys lactate concentrations in healthy rats. In this study, 48 rats were divided into 4 groups (baseline [GVB] , Ac, EAc10Hz and EAc100Hz) and subdivided into 2 subgroups according to the time of samples collection (T-30, immediately after the end of the session or Ac or EAc and T-60: 30 minutes after the end of the session). All rats were anesthetized and experimental groups (Ac, EAc10Hz and EAc100Hz) were submitted respectively to the EAc and Ac (10 Hz and 100 Hz) on acupoints ST-36 and RM-12 for 30 minutes. Glucose, lactate and LDH were assayed in the blood. Tissue lactate concentration was assayed in liver and kidney of rats at T-30 and T-60 timepoints. Stimulation of acupoints ST-36 and RM-12 by EAc 10Hz significantly reduced blood glucose levels in healthy rats, at the end of electrical stimulation (190.80 Â 24.88 vs. 138.50 Â 21.09, p> 0.001) and 30 minutes later (186.30 Â 23.76 vs. 134.10 Â 25.65 , p> 0.001). Using a frequency 10 times higher (100Hz) similar results were obtained (T-30, 186.30 Â 23.76 vs. 99.17 Â 8.79, p> 0.001, and T-60, 190.80 Â 24.88 vs. 113.40 Â 7.37, p> 0.001). Ac stimulation did not significantly alter blood glucose.
There was also a significant reduction in the concentrations of LDH immediately after the
acupuncture session and 30 minutes later, both in animals subjected to Ac (T-30, 518.20 Â
43.31 vs. 354.80 Â 44.66, p <0.001, T-60, 637.80 Â 61.01 vs. 325.70 Â 10.54, p <0.001) and EAc (10Hz: T-30, 518.20 Â 43.31 vs. 286.50 Â 59.86, p <0.001, T-60, 637.80 Â 61.01 vs 268.80 Â 43.51, p <0.001 and 100Hz: T-30, 518.20 Â 43.31 vs. 213, 80 Â 24.85, p <0.001, T- 60, 637.80 Â 61.01 vs 188.20 Â 24.00, p <0.001), suggesting a lower anaerobic metabolism. In this study, a significant decrease in serum lactate 30 minutes after the start of the study in rats subjected to EAc 100 Hz suggests the prevalence of aerobic glycolysis (2.10 Â 0.23 vs. 1.19 Â 1.02, p < 0.001). Similar result was observed in the levels of lactate in the liver (T-60, 10 Hz: 1.92 Â 0.42 vs. 0.69 Â 0.29, p <0.001 and 100 Hz: 1.92 Â 0.42 vs. 0.44 Â 0.21, p <0.001) and kidney (T-30, 100 Hz: 1.86 Â 0.87 vs. 0.91 Â 0.38, p <0.05). The results of this study show that the Ac and EAc, can reduce the concentrations of blood glucose in hyperglycemic animals. Higher frequencies have greater hypoglycemic effect. The reduction of blood glucose should be a consequence of the action of endogenous opioids and - endorphin, as suggested in previous studies. The mechanism of reduction of lactate and LDH appears to be related to a prevalence of aerobic glycolysis.
| Identifer | oai:union.ndltd.org:IBICT/oai:www.teses.ufc.br:4221 |
| Date | 21 December 2009 |
| Creators | Lanesse Medeiros de Figueiredo |
| Contributors | SÃrgio Botelho GuimarÃes, Maria dos Desterro Leiros, Maria Luzete Costa Cavalcante |
| Publisher | Universidade Federal do CearÃ, Programa de PÃs-GraduaÃÃo em Cirurgia, UFC, BR |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFC, instname:Universidade Federal do Ceará, instacron:UFC |
| Rights | info:eu-repo/semantics/openAccess |
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