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DNA strand breaks in crustaceans as an indicator of marine pollution.

Chan Kwan-ling. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (leaves 91-105). / Abstracts in English and Chinese. / Abstract --- p.i / Acknowledgements --- p.v / Contents --- p.vi / List of figures and tables --- p.ix / Chapter Chapter 1 --- Introduction / Chapter 1.1 --- Literature review --- p.1 / Chapter 1.1.1 --- The effect of pollutants on the genetic materials of aquatic organisms --- p.1 / Chapter 1.1.1.1 --- Response of individual to genotoxicants --- p.1 / Chapter 1.1.1.2 --- Effects of genotoxicants on population structure --- p.3 / Chapter 1.1.2 --- Application of genetic markers in monitoring water pollution --- p.3 / Chapter 1.1.2.1 --- DNA adduct --- p.4 / Chapter 1.1.2.2 --- Sister chromatid exchange (SCE) test --- p.5 / Chapter 1.1.2.3 --- Micronucleus --- p.6 / Chapter 1.1.2.4 --- DNA strand breaks --- p.7 / Chapter 1.1.3 --- Single-cell gel electrophoresis (comet) assay --- p.9 / Chapter 1.1.4 --- Test organisms for comet assay --- p.12 / Chapter 1.2 --- Objective of the present study --- p.13 / Chapter Chapter 2 --- Genotoxicity of pollutants on Hyale crassicornis / Chapter 2.1 --- Introduction --- p.22 / Chapter 2.2 --- Materials and methods --- p.24 / Chapter 2.2.1 --- Sampling of amphipods --- p.24 / Chapter 2.2.2 --- Acclimation --- p.24 / Chapter 2.2.3 --- Acute toxicity test --- p.26 / Chapter 2.2.4 --- The effect of test duration on DNA damage --- p.27 / Chapter 2.2.5 --- Effect of toxicants on DNA damage --- p.28 / Chapter 2.2.6 --- Comet assay --- p.29 / Chapter 2.2.7 --- Chemicals --- p.34 / Chapter 2.2.8 --- Data analysis --- p.34 / Chapter 2.3 --- Results --- p.34 / Chapter 2.4 --- Discussion --- p.47 / Chapter Chapter 3 --- Genotoxicity of hydrogen peroxide on different tissue types of Metapenaeus ensis / Chapter 3.1 --- Introduction --- p.54 / Chapter 3.2 --- Materials and Methods --- p.57 / Chapter 3.2.1 --- Collection and acclimation of shrimps --- p.57 / Chapter 3.2.2 --- Incubation --- p.59 / Chapter 3.2.3 --- Comet Assay --- p.60 / Chapter 3.2.4 --- Chemicals --- p.61 / Chapter 3.2.5 --- Data analysis --- p.61 / Chapter 3.3 --- Results --- p.61 / Chapter 3.4 --- Discussion --- p.67 / Chapter Chapter 4 --- Genotoxicity of wastewater on Hyale crassicornis / Chapter 4.1 --- Introduction --- p.71 / Chapter 4.2 --- Materials and Methods --- p.72 / Chapter 4.2.1 --- Collection of wastewater samples --- p.72 / Chapter 4.2.2 --- Metal content analysis --- p.73 / Chapter 4.2.3 --- Genotoxic effect of wastewater samples on Hyale crassicornis --- p.74 / Chapter 4.2.4 --- Chemicals --- p.76 / Chapter 4.2.5 --- Statistical analysis --- p.77 / Chapter 4.3 --- Results --- p.77 / Chapter 4.3.1 --- Metals content in water samples --- p.77 / Chapter 4.3.2 --- DNA damage --- p.79 / Chapter 4.4 --- Discussion --- p.79 / Chapter Chapter 5 --- Conclusions --- p.89 / References --- p.91

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_325132
Date January 2005
ContributorsChan, Kwan-ling., Chinese University of Hong Kong Graduate School. Division of Biology.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xi, 105 leaves : ill. (some col.) ; 30 cm.
CoverageChina, Hong Kong, China, Hong Kong, China, Hong Kong
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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