Made available in DSpace on 2014-12-17T14:03:41Z (GMT). No. of bitstreams: 1
TaffarelMT_DISSERT.pdf: 2213102 bytes, checksum: 779f70cd3a9747e4f3a386630bd3d1cc (MD5)
Previous issue date: 2013-04-09 / Funda??o de Apoio ? Pesquisa do Estado do Rio Grande do Norte / Oilseeds are a high-value natural resource, due to its use as a substitute for
petroleum. However, the storage time can reduce seed viability and oil quality.
Therefore, scientific efforts have been made to provide a increment of storage time,
germination rates and plant establishment of high-value oilseeds. The seedling
establishment depends of the plant pass over the functional transition stage,
characterized by a metabolic change from heterotrophic condition to autotrophic one.
The storage oil mobilization is performed by β-oxidation process and the glyoxylate
cycle. Also, the functional transition involves acclimation to photosynthetic condition,
which generally includes the participation of antioxidant system and the reactive
oxygen species, the latter are produced in various reactions of primary and
secondary metabolism. In the present study, Catalase was inhibited during the
functional transition of sunflower and safflower, after were performed many analyzes
to elucidate the effects caused on the SOD and APX antioxidant systems. Also, were
checked the changes in expression pattern of the glyoxylate cycle enzymes markers,
ICL and MLS. It was observed that after CAT inhibition, the SOD and APX antioxidant
systems allow the seedling establishment. Besides, was verified that both oilseeds
can be accelerate the reverse mobilization and the photosynthetic establishment
when Catalase activity has dramatically decreased / As sementes oleaginosas armazenam ?leos, um recurso natural de alto valor
econ?mico devido a sua aplicabilidade como substituto aos derivados do petr?leo.
Por?m, o tempo de armazenamento pode inviabilizar a semente provocando a
redu??o da qualidade do ?leo e perda da viabilidade da semente. Por este motivo,
esfor?os cient?ficos t?m sido realizados para proporcionar um maior tempo de
armazenamento e incrementar taxas de germina??o e estabelecimento destas
plantas com alto valor econ?mico. O estabelecimento da pl?ntula depende da
capacidade do vegetal transpassar a etapa de transi??o funcional, caracterizada
pela mudan?a do estado heterotr?fico para o autotr?fico. O consumo das reservas
de ?leo depende das vias de β-oxida??o e do ciclo do glioxilato. No entanto, o
processo de transi??o envolve a aclimata??o do vegetal ? condi??o de organismo
fotossintetizante, que em geral conta com a participa??o fundamental do sistema
antioxidante do vegetal devido ? produ??o de esp?cies reativas de oxig?nio em
v?rias rea??es do metabolismo prim?rio e secund?rio. Neste estudo, a Catalase foi
inibida durante a transi??o funcional de girassol e c?rtamo para analisar os efeitos
provocados nos sistemas antioxidantes de APX e da SOD e verificar as
modifica??es no padr?o de express?o das enzimas marcadoras do ciclo do
glioxilato, ICL e MLS. Constatou-se que diante da inibi??o da Catalase o sistema
antioxidante da APX e da SOD permitem o estabelecimento da pl?ntula. Verificou-se
tamb?m que as duas oleaginoasas parecem acelerar a mobiliza??o de reservas e o
estabelecimento fotossint?tico quando a Catalase tem a atividade drasticamente
reduzida
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/12608 |
| Date | 09 April 2013 |
| Creators | Torres, Taffarel Melo |
| Contributors | CPF:79332021368, http://lattes.cnpq.br/3289758851760692, Lanza, Daniel Carlos Ferreira, CPF:05372896663, http://lattes.cnpq.br/6851351991421755, Cunha, Rodrigo Maranguape Silva da, CPF:70290008387, http://lattes.cnpq.br/1918056067943429, Lima, Jo?o Paulo Matos Santos |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Bioqu?mica, UFRN, BR, Bioqu?mica; Biologia Molecular |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
Page generated in 0.0022 seconds