G-Protein Coupled Receptors (GPCRs) are a class of membrane-bound receptor proteins capable of relaying a biological signal across a cell membrane through a solely conformational change in their transmembrane domain. Previous work has shown that helical foldamers composed of achiral monomeric units can be used in an analogous manner to relay stereochemical information on the nano-scale through the conformational control of screw-sense preference. While this work has produced some highly successful examples of signal relay, mimicking the function of GPCRs, its reliance on screw-sense responsive NMR probes has restricted further development into membrane environments. This thesis describes the successful development of a pyrene based screw-sense responsive fluorescence probe and its subsequent use in the development of a series of membrane-based GPCR mimics. This thesis has also details the preliminary steps towards the development of light-responsive controllers of screw-sense preference for nano-scale signal relay devices.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:689550 |
| Date | January 2015 |
| Creators | Lister, Francis George Alexander |
| Publisher | University of Manchester |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | https://www.research.manchester.ac.uk/portal/en/theses/fluorescent-probes-of-conformational-signal-relay-in-membrane-environments(baa2cddd-94a1-4505-bf45-13403e0a6548).html |
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