Lai Kit Ching Jan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 121-141). / Abstracts in English and Chinese. / Abstract --- p.i / 摘要 --- p.iv / Acknowledgements --- p.vi / List of abbreviations --- p.vii / List of figures --- p.ix / Table of contents --- p.xi / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- "The structure, function and regulation of growth hormone" --- p.1 / Chapter 1.2 --- Historical perspective of growth hormone secretagogues --- p.5 / Chapter 1.3 --- Ghrelin and growth hormone secretagogue receptor --- p.7 / Chapter 1.4 --- Calcium signalling of growth hormone secretagogues --- p.12 / Chapter 1.5 --- Pancreas and functions of exocrine pancreas --- p.15 / Chapter 1.6 --- Regulation of exocrine pancreatic function --- p.22 / Chapter 1.7 --- Functions of ghrelin in pancreas --- p.32 / Chapter 1.8 --- Aims of study --- p.34 / Chapter Chapter 2 --- Materials and methods --- p.35 / Chapter 2.1 --- Experimental animal and cell line models --- p.35 / Chapter 2.1.1 --- Rat model --- p.35 / Chapter 2.1.2 --- Isolation of pancreatic lobules and acinar cells --- p.35 / Chapter 2.1.3 --- Omeprazole-induced gastric acid inhibition --- p.36 / Chapter 2.1.4 --- Cerulein-induced acute pancreatitis --- p.37 / Chapter 2.1.5 --- Starvation rat model --- p.37 / Chapter 2.1.6 --- AR42J cell line --- p.38 / Chapter 2.2 --- Reverse transcriptase-polymerase chain reaction --- p.39 / Chapter 2.2.1 --- Total RNA extraction and quantification --- p.39 / Chapter 2.2.2 --- Reverse transcription --- p.40 / Chapter 2.2.3 --- Polymerase chain reaction --- p.40 / Chapter 2.2.4 --- Gel electrophoresis --- p.41 / Chapter 2.2.5 --- Optimization of semi-quantitative RT-PCR --- p.42 / Chapter 2.3 --- Western blot analysis --- p.43 / Chapter 2.3.1 --- Extraction of total protein and quantification --- p.43 / Chapter 2.3.2 --- Sodium Dodecyl-sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) --- p.43 / Chapter 2.3.3 --- Tricine Sodium Dodecyl-sulphate Polyacrylamide Gel Electrophoresis (Tricine-SDS-PAGE) --- p.44 / Chapter 2.3.4 --- Electroblotting and immunodetection of proteins --- p.45 / Chapter 2.4 --- Immunocytochemistry --- p.47 / Chapter 2.4.1 --- Preparation of isolated acinar cells for paraffin sections --- p.47 / Chapter 2.4.2 --- Preparation of AR42J cells slices --- p.47 / Chapter 2.4.3 --- Immunofluorescent double staining --- p.47 / Chapter 2.5 --- Functional studies of digestive enzyme secretion from pancreatic isolated acini and lobules --- p.49 / Chapter 2.5.1 --- Incubation of pancreatic isolated acini and lobules with different peptides --- p.49 / Chapter 2.5.2 --- Quantification of protein content --- p.50 / Chapter 2.5.3 --- Measurement of a-amylase secretion by α-amylase assay --- p.50 / Chapter 2.6 --- Spectrofluorimetric measurement --- p.52 / Chapter 2.6.1 --- Incubation of AR42J cells with Fluo-4/AM --- p.52 / Chapter 2.6.2 --- Pretreatment of antagonist or blockers and Ca2+-free treatment --- p.52 / Chapter 2.6.3 --- Calcium mobilization assay --- p.53 / Chapter 2.7 --- Statistics and data analysis --- p.54
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324984 |
| Date | January 2004 |
| Contributors | Lai, Kit Ching Jan., Chinese University of Hong Kong Graduate School. Division of Physiology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xii, 141 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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