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Previous issue date: 2017-12-12 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Lippia (Verbenaceae) is a plant genus distributed throughout all Southern and Central America, and Tropical Africa. Approximately 60 species are endemic in Brazil and 22 are endemic in the state of Bahia. Lippia has a long history of use in traditional medicine, the most common use being in the treatment of respiratory diseases. Despite Lippia?s extensive history in treating diseases, little is known about its anticancer properties against brain and central nervous system tumors. This paper explores the anticancer potential of 5 species of Lippia from the state of Bahia (Brazil),Lippia alnifolia, Lippia insignis, Lippia lasiocalycina, Lippia origanoides and Lippia thymoides. To assess the cell viability of essential oils and methanol extracts derived from these species MTT assay was utilized. To assess the effects of the essential oils and methanol extracts on morphology of cancer cells a Nikon Ts-100 phase contrast microscope was used, and photos were taken with a Nikon E4300. All essential oils were evaluated at 0.1 mg.mL-1, 0.3 mg.mL-1, 0.5 mg.mL-1, and 1 mg.mL-1. All methanol extracts were evaluated at 1 ?g.mL-1, 10 ?g.mL-1, 100 ?g.mL-1, 500 ?g.mL-1, and 1000 ?g.mL-1. The ability to inhibit cell migration was assessed by wound heal assay. All methanol extracts were evaluated at 500 ?g.mL-1. All essential oils were evaluated at 0.1 mg.mL-1. To access the effects of essential oils and methanol extracts on the cell cycle flow cytometry was used. All methanol extracts were evaluated at 1000 ?g.mL-1. All essential oils were evaluated at 0.03 mg.mL-1. The essential oils derived from Lippia alnifolia, Lippia insignis, Lippia lasiocalycina, and Lippia origanoides all drastically decreased the cell viability of the C6 cell line in a concentration dependent manner. The essential oil derived from Lippia thymoides increased cell viability in a concentration dependent manner. All of the methanol extracts appear to have the ability to abruptly decrease cell viability at either 500 ?g.mL-1 or 1000 ?g.mL-1 it. Lippia alnifolia and Lippia insignis both have the ability to significantly alter the morphology of the C6 cell line. Methanol extracts derived from Lippia alnifolia, Lippia insignis, Lippia lasiocalycina, Lippia origanoides and Lippia thymoides all significantly inhibit the migration of C6 cells at a concentration of 500 ?g.mL-1. Essential oils obtained from Lippia alnifolia, Lippia insignis, Lippia lasiocalycina, Lippia origanoides and Lippia thymoides significantly inhibited the migration of C6 cells at a concentration of 0.1 mg.mL-1. All methanol extracts and essential oils tested demonstrated the ability to induce some degree of nuclear fragmentation, vacuolization, and cytoplasmic swelling. These results indicate that the constituents of both the essential oils and methanol extracts should be isolated and their anticancer potential should be further explored. / Lippia (Verbenaceae) ? um g?nero de planta distribu?do pela Am?rica do Sul, Central e a ?frica Tropical. Aproximadamente 60 esp?cies s?o end?micas do Brasil e 22 s?o end?micas do estado da Bahia. Lippia tem uma longa hist?ria de uso na medicina tradicional, sendo o uso mais comum no tratamento de doen?as respirat?rias. Apesar da extensa hist?ria de Lippia no tratamento de doen?as, pouco se sabe sobre suas propriedades antitumorais contra tumores cerebrais. Este estudo buscou explorar o potencial antiglioma de ?leos essenciais e extratos metan?licos de Lippia alnifolia, Lippia insignis, Lippia lasiocalycina, Lippia origanoides e Lippia thymoides em c?lulas de glioma da linhagem C6. Para avaliar a viabilidade celular foram utilizados o ensaio do MTT. Os efeitos das amostras na morfologia de c?lulas cancerosas foram avaliados utilizando-se um microsc?pio de contraste de fase Nikon Ts-100 e fotos com uma Nikon E4300. Os ?leos essenciais foram avaliados nas concentra??es de 0,1 mg.mL-1, 0,3 mg.mL-1, 0,5 mg.mL-1 e 1 mg.mL-1 e os extratos de metanol a 1 ?g.mL-1, 10 ?g.mL-1, 100 ?g.mL-1, 500 ?g.mL-1 e 1000 ?g.mL-1. A capacidade de inibir a migra??o celular foi avaliada pelo ensaio de cicatriza??o da ferida, na qual os extratos foram avaliados a 500 ?g.mL-1 e os ?leos essenciais a 0,1 mg.mL-1. O ciclo celular foi avaliado na citometria de fluxo nas concentra??es de 1000 ug.mL-1 para os extratos e 0,03 mg.mL-1 para os ?leos essenciais. Os ?leos essenciais obtidos de Lippia alnifolia, Lippia insignis, Lippia lasiocalycina e Lippia origanoides reduziram drasticamente a viabilidade celular C6 de uma maneira concentrac?o-dependente. J? o ?leo essencial de Lippia thymoides aumentou a viabilidade celular de uma maneira concentrac?o-dependente. Todos os extratos de metanol parecem ter a capacidade de diminuir abruptamente a viabilidade celular em 500 ?g.mL-1 ou 1000 ?g.mL-1. Lippia alnifolia e Lippia insignis parecem ter a capacidade de alterar significativamente a morfologia da linha celular C6. Os extratos metan?licos de Lippia alnifolia, Lippia insignis, Lippia lasiocalycina, Lippia origanoides e Lippia thymoides inibem significativamente a migra??o de c?lulas C6 a uma concentra??o de 500 ?g.mL-1. Os ?leos essenciais de Lippia alnifolia, Lippia insignis, Lippia lasiocalycina, Lippia origanoidese Lippia thymoides inibem significativamente a migra??o de c?lulas C6 a uma concentra??o de 0,1 mg.mL-1. Todos os extratos metan?licos e ?leos essenciais testados demonstraram a capacidade de induzir algum grau de fragmenta??o nuclear, vacuoliza??o e incha?o citoplasm?tico.Estes resultados indicam que os constituintes dos ?leos essenciais e extratos metan?licos devem ser isolados e seu potencial anticancer?geno deve ser explorado.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.uefs.br:8080:tede/713 |
| Date | 12 December 2017 |
| Creators | Paige, Eric-Lenn Michael |
| Contributors | Lucchese, Ang?lica Maria |
| Publisher | Universidade Estadual de Feira de Santana, Mestrado Acad?mico em Recursos Gen?ticos Vegetais, UEFS, Brasil, DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UEFS, instname:Universidade Estadual de Feira de Santana, instacron:UEFS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 8146646481622241874, 600, 600, 600, 600, 5026123383450589282, -3091138714907603907, 3590462550136975366 |
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