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Identification of odorous compounds in commercial chaw tofu and evaluation of the quality of model broths during fermentation.

Cheung Hiu-Ming. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (leaves 140-150). / Abstracts in English and Chinese. / ABSTRACT --- p.i / ACKNOWLEDGEMENTS --- p.vi / LIST OF FIGURES --- p.xiii / LIST OF TABLES --- p.xv / Chapter CHAPTER 1 --- LITERATURE REVIEW --- p.1 / Chapter 1.1 --- Introduction --- p.2 / Chapter 1.2 --- Soybeans --- p.3 / Chapter 1.2.1 --- Chemistry and nutritional value of soybeans --- p.3 / Chapter 1.2.2 --- Protein composition of soybeans --- p.4 / Chapter 1.2.3 --- Volatile compounds in soybeans --- p.4 / Chapter 1.3 --- Food fermentation --- p.5 / Chapter 1.4 --- Chaw tofu --- p.6 / Chapter 1.4.1 --- Preparation of tofu --- p.7 / Chapter 1.4.2 --- Preparation of chaw tofu --- p.7 / Chapter 1.4.3 --- Microorganisms involved in fermentation of chaw tofu --- p.8 / Chapter 1.4.4 --- Volatile components in chaw tofu --- p.11 / Chapter 1.4.5 --- Proteolytic activity of chaw tofu --- p.12 / Chapter 1.5 --- Stinky brine broth --- p.13 / Chapter 1.5.1 --- The pH value of stinky brine broth --- p.13 / Chapter 1.5.2 --- The salt percentage of stinky brine broth --- p.14 / Chapter 1.5.3 --- Volatile components of stinky brine broth --- p.14 / Chapter 1.5.4 --- Parameters affecting ammonia production of stinky brine --- p.15 / Chapter 1.6 --- Other fermented soy products --- p.16 / Chapter 1.6.1 --- Microorganisms involved in the fermentation --- p.16 / Chapter 1.6.1.1 --- Fermentation of soybean by bacteria --- p.17 / Chapter 1.6.1.1.1 --- Natto --- p.17 / Chapter 1.6.1.1.2 --- Kinema --- p.18 / Chapter 1.6.1.1.3 --- Soy daddawa --- p.19 / Chapter 1.6.1.1.4 --- Hawaijar --- p.20 / Chapter 1.6.1.1.5 --- Thua nao --- p.21 / Chapter 1.6.1.2 --- Fermentation of soybean by moulds --- p.21 / Chapter 1.6.1.2.1 --- Tempe --- p.21 / Chapter 1.6.1.2.2 --- Sufu --- p.22 / Chapter 1.6.1.2.3 --- Soy sauce --- p.22 / Chapter 1.6.1.2.4 --- Soy paste --- p.23 / Chapter 1.6.2 --- Formation of volatile compounds during Bacillus fermentation --- p.24 / Chapter 1.6.3 --- Biochemical changes during fermentation --- p.21 / Chapter 1.7 --- Methods of flavor analysis --- p.30 / Chapter 1.7.1 --- Headspace Analysis --- p.31 / Chapter 1.7.2 --- Aroma characterization --- p.32 / Chapter CHAPTER 2 --- IDENTIFICATION OF ODOROUS COMPOUNDS IN COMMERCIAL CHAW TOFU BASED ON ODOR ACTIVITY EVALUATION --- p.42 / Chapter 2.1 --- Introduction --- p.43 / Chapter 2.2 --- Materials & Methods --- p.46 / Chapter 2.2.1 --- Experimental samples --- p.46 / Chapter 2.2.2 --- Headspace-Gas Chromatography-Mass Spectrometry (GC-MS) --- p.46 / Chapter 2.2.3 --- Conditions of the Gas Chromatography-Mass Spectrometry (GC-MS) --- p.47 / Chapter 2.2.4 --- Compound identifications --- p.48 / Chapter 2.2.5 --- Quantification of compounds --- p.48 / Chapter 2.2.6 --- Statistical analysis --- p.48 / Chapter 2.2.7 --- Calculation of odor activity value (OAV) --- p.49 / Chapter 2.3 --- Results & Discussion --- p.50 / Chapter 2.3.1 --- Odor activity value (OAV) --- p.51 / Chapter 2.3.2 --- Volatile compounds in fresh samples --- p.51 / Chapter 2.3.2.1 --- Comparison of odorous compounds in fresh samples among different locations --- p.52 / Chapter 2.3.3 --- Volatile compounds in deep-fat fried samples --- p.53 / Chapter 2.3.3.1 --- Comparison of odorous compounds in deep-fat fried samples among different locations --- p.54 / Chapter 2.3.4 --- Comparison between fresh and deep-fat fried samples --- p.55 / Chapter 2.3.5 --- Odorous compounds of chaw tofu based on OAVs --- p.56 / Chapter 2.3.6 --- Possible ways for formation of odorous compounds --- p.58 / Chapter 2.3.6.1 --- Protein degradation --- p.58 / Chapter 2.3.6.2 --- Lipid degradation --- p.59 / Chapter 2.3.7 --- Comparison between volatile compounds in chaw tofu and others fermented soybean products --- p.60 / Chapter 2.4 --- Conclusion --- p.61 / Chapter CHAPTER 3 --- IDENTIFICATION OF ODOROUS COMPOUNDS IN COMMERCIAL CHAW TOFU BASED ON GAS CHROMATOGRAPHY-OLFACTOMETRY --- p.67 / Chapter 3.1 --- Introduction --- p.68 / Chapter 3.2 --- Materials & Methods --- p.71 / Chapter 3.2.1 --- Experimental samples --- p.71 / Chapter 3.2.2 --- Gas Chromatography-Mass Spectrometry-Flame Ionization Detection-Olfactometry (GC-MS-FID-O) --- p.71 / Chapter 3.2.3 --- Conditions of the Gas Chromatography-Mass Spectrometry --- p.72 / Chapter 3.2.4 --- Detection of odor active compounds --- p.73 / Chapter 3.2.5 --- Compound identifications --- p.73 / Chapter 3.3 --- Results & Discussion --- p.74 / Chapter 3.3.1 --- "Fecal, rancid and putrid odor" --- p.74 / Chapter 3.3.2 --- "Cabbages, sulfurous and meaty odor" --- p.76 / Chapter 3.3.3 --- Green odor --- p.77 / Chapter 3.3.4 --- Other odor contributing compounds --- p.77 / Chapter 3.3.5 --- Odor generate during deep-fat frying --- p.78 / Chapter 3.3.6 --- Comparison between GC-O and OAVs --- p.79 / Chapter 3.3.7 --- Comparison between volatile compounds in chaw tofu and others fermented soybean products --- p.80 / Chapter 3.4 --- Conclusion --- p.82 / Chapter CHAPTER 4 --- EVALUATION OF CHAW TOFU MODEL FERMENTATION BROTH --- p.86 / Chapter 4.1 --- Introduction --- p.87 / Chapter 4.2 --- Materials & Methods --- p.90 / Chapter 4.2.1 --- Model fermentation broth preparation --- p.90 / Chapter 4.2.2 --- Tofu sample preparation --- p.91 / Chapter 4.2.3 --- Gas Chromatography-Mass Spectrometry --- p.91 / Chapter 4.2.3.1 --- Conditions of Gas Chromatography-Mass Spectrometry (GC-MS) --- p.92 / Chapter 4.2.3.2 --- Compound identification --- p.93 / Chapter 4.2.3.3 --- Quantification of compounds --- p.93 / Chapter 4.2.4 --- Viable cell counts --- p.93 / Chapter 4.2.5 --- pH value and soluble content --- p.94 / Chapter 4.2.6 --- Proteolytic activity --- p.94 / Chapter 4.2.7 --- Statistical analysis --- p.95 / Chapter 4.3 --- Results & Discussion --- p.96 / Chapter 4.3.1 --- Headspaces analysis --- p.96 / Chapter 4.3.1.1 --- Changes in volatile composition in model fermentation broths --- p.97 / Chapter 4.3.1.2 --- Comparison of volatile compositions between the broths --- p.98 / Chapter 4.3.1.3 --- Comparison of volatile compositions among the three deep-fat fried fermented tofu with different broths --- p.101 / Chapter 4.3.1.4 --- Comparison of volatile compositions of deep fat fried fermented tofu with that of the commercial chaw tofu --- p.102 / Chapter 4.3.2 --- Liquid samples analysis --- p.104 / Chapter 4.3.2.1 --- "Changes in viable cell counts, pH values, protease activities and soluble solid contents within model fermentation broths during fermentation" --- p.106 / Chapter 4.3.2.2 --- Viable cell counts --- p.107 / Chapter 4.3.2.3 --- Soluble solid content --- p.108 / Chapter 4.3.2.4 --- Proteolytic activity --- p.106 / Chapter 4.3.2.5 --- pH value --- p.110 / Chapter 4.4 --- Conclusion --- p.112 / Chapter CHAPTER 5 --- GENERAL CONCLUSION --- p.127 / APPENDIX --- p.130 / IDENTIFICATION OF MICROORGANISMS PRESENTED IN THE MODEL CHAW TOFU FERMENTATION BROTHS BY MICROBIAL IDENTIFICATION SYSTEM (MIDI) --- p.130 / Materials & Methods --- p.130 / Model fermentation broth preparation --- p.130 / Viable cell counts --- p.131 / Microbial Identification System (MIDI) --- p.131 / Results --- p.133 / Suggestion on further investigation --- p.134 / REFERNECES --- p.141

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_325116
Date January 2005
ContributorsCheung, Hiu-Ming., Chinese University of Hong Kong Graduate School. Division of Biology.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xvi, 150 leaves : ill. ; 30 cm.
CoverageChina
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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