The aim of thesis was to study the effect of fluoranthene (FLT) on growth and biochemical processes of plant Petunia (Petunia hybrida cv. Lady blue) in in vitro conditions. In this experiment were used segments of the middle parts of the leaves, shoots from organogenesis in vitro and apical meristem of stem with four to five leaves from long-term in vitro culture. It was observed if fluoranthene influence organogenesis of shoots in vitro, contents of chlorophyll a and b, content of cytokinins and rating of shoots (from organogenesis in vitro) and apical meristem of stem with four to five leaves. It was used culture MS medium containing benzylaminopurin (1.5 mg/l), 1-naphtylacetic acid (0.18 mg/l) and fluoranthene (1 mg/l) or without fluoranthene (control). In the case of organogenesis in vitro, the experiment was performed at 4 different variations of media with subculturing to other media in triplicate. Variations of the media were chosen to allow period of cultivation with fluoranthene, which affects growth and biochemical processes. MS medium containing indol-3-butyric acid (0.5 mg/l) and FLT in different concentrations (0.1, 1, 3 a 5 mg/l) was used for monitoring rooting. The results showed that fluoranthen is able to affect organogenesis of shoots formed on the segments of leaves. There was lower frequency compared to the control organogenesis. It was found that fluoranthene more affects beginning of cultivation compared to later exposure of fluoranthene during cultivation. It was found the highest uptake of benzyladenine and its riboside from the culture medium. Fluoranthene affected significantly content of dihydrozeatin. Zeatin and its riboside were recorded in a minimal amount. Fluoranthene caused lower content of chlorophylls a and b in explantats. In the case of chlorophyll a were no significant differences between influence by fluoranthene at the beginning of the cultivation or during of cultivation. It was noted that fluoranthene influence the morphology of the roots subcultured shoots from organogenesis in vitro. Plant roots exhibited less branching and roots were shorter in culture medium containing higher content of fluoranthene (3 and 5 mg/l).
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:178912 |
| Date | January 2014 |
| Creators | Czyžová, Kateřina |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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