Duan Lifang. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 83-87). / Abstracts in English and Chinese. / Title Page --- p.i / Abstract (English) --- p.ii / Abstract (Chinese) --- p.iii / Acknowledgements --- p.iv / Table of Contents --- p.v / List of Tables --- p.vii / List of Figures --- p.viii / Symbols and Abbreviations --- p.x / Chapter CHAPTER 1 --- INTRODUCTION / Chapter 1.1 --- Mass spectrometry in biochemistry --- p.1 / Chapter 1.2 --- Electrospray ionization (ESI) --- p.2 / Chapter 1.3 --- Fourier transforms ion cyclotron resonance mass spectrometry (FTICR-MS) --- p.4 / Chapter 1.3.1 --- Ion motions --- p.5 / Chapter 1.3.2 --- Mass calibration --- p.7 / Chapter 1.3.3 --- Tandem mass spectrometry --- p.11 / Chapter 1.4 --- Sustained off-resonance irradiation collision-induced dissociation (SORI-CID) of peptide ions --- p.13 / Chapter 1.5 --- Outline of present work --- p.18 / Chapter CHAPTER 2 --- INSTRUMENTATION AND EXPERIMENTAL / Chapter 2.1 --- Fourier-trans form ion cyclotron resonance mass spectrometer --- p.19 / Chapter 2.1.1 --- Vacuum system --- p.19 / Chapter 2.1.2 --- Nanospray source --- p.23 / Chapter 2.1.3 --- Ion transfer system --- p.26 / Chapter 2.1.4 --- Infinity cell --- p.28 / Chapter 2.1.5 --- Data acquisition system --- p.29 / Chapter 2.2 --- Experimental --- p.29 / Chapter 2.2.1 --- Simple acquisition pulse program --- p.30 / Chapter 2.2.2 --- Tandem mass spectrometry (SORI-CID MS/MS) --- p.32 / Chapter CHAPTER 3 --- DISSOCIATION OF MODEL PEPTIDE IONS UNDER SORI-CID CONDITIONS / Chapter 3.1 --- Introduction --- p.35 / Chapter 3.2 --- Experimental --- p.37 / Chapter 3.3 --- Results and discussion --- p.41 / Chapter 3.3.1 --- "SORI-CID of XG3WG3X (where X 二 R, K and N)" --- p.41 / Chapter 3.3.2 --- SORI-CID of RGnWGnR (where n=l -5) --- p.46 / Chapter 3.3.3 --- SORI-CID of RG3XG3R (where X = D and E) --- p.48 / Chapter 3.4.4 --- SORI-CID of sequence isomers of RG5WG5R --- p.51 / Chapter 3.4 --- Conclusions --- p.56 / Chapter CHAPTER 4 --- EXACT MASS MEASUREMENTS OF THE PRODUCT IONS DERIVED FROM SORI-CID FTICR-MS / Chapter 4.1 --- Introduction --- p.57 / Chapter 4.2 --- Experimental --- p.60 / Chapter 4.3 --- Results and discussion --- p.61 / Chapter 4.3.1 --- Origin(s) of the mass measurement error --- p.63 / Chapter 4.3.2 --- Pressure effect on the cyclotron frequencies of trapped ions --- p.67 / Chapter 4.3.3 --- Modified internal lock-mass calibration method --- p.70 / Chapter 4.4 --- Conclusions --- p.79 / Chapter CHAPTER 5 --- CONCLUSIONS --- p.81 / REFERENCES --- p.83 / APPENDIX I The Proton affinity (PA) and the structural formulae of 20 α-amino acids --- p.88 / APPENDIX II Schematics of the proposed mechanisms of peptide dissociation under low-energy CID conditions --- p.89 / APPENDIX III Pulse programs for simple MS and MS/MS experiments --- p.92 / APPENDIX IV Schematics of the proposed mechanisms for the formation of y8+ ions from the singly protonated arginine- and lysine-containing peptides --- p.99
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324347 |
| Date | January 2003 |
| Contributors | Duan, Lifang., Chinese University of Hong Kong Graduate School. Division of Chemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, x, 100 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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