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Differentially expressed genes during fruit body development of Shiang-gu mushroom, Lentinula edodes.

by Xie Weijun. / Publication date from spine. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1995. / Includes bibliographical references (leaves 107-122). / ABSTRACT --- p.i / ACKNOWLEDGMENTS --- p.iii / TABLE OF CONTENTS --- p.iv / LIST OF FIGURES --- p.vii / LIST OF TABLES --- p.x / Chapter 1. --- INTRODUCTION --- p.1 / Chapter 2. --- LITERATURE REVIEW --- p.4 / Chapter 2.1 --- Economic and biotechnological significance of Lentinula edodes --- p.4 / Chapter 2.2 --- Biological background --- p.8 / Chapter 2.2.1 --- Life cycle --- p.8 / Chapter 2.2.2 --- Morphological changes during fruit body development --- p.9 / Chapter 2.2.3 --- Biochemistry of fruit body development --- p.13 / Chapter 2.2.4 --- Molecular studies --- p.17 / Chapter 2.3 --- RNA AP-PCR --- p.23 / Chapter 3. --- MATERIALS AND METHODS --- p.26 / Chapter 3.1 --- Biological materials --- p.26 / Chapter 3.2 --- Media --- p.26 / Chapter 3.3 --- RNA AP-PCR with RNAs isolated from four different developmental stages --- p.27 / Chapter 3.3.1 --- Isolation of total RNAs --- p.27 / Chapter 3.3.2 --- RNA AP-PCR --- p.30 / Chapter 3.3.3 --- PCR reamplification --- p.33 / Chapter 3.4 --- PCR cloning and screening --- p.33 / Chapter 3.4.1 --- PCR product purification --- p.34 / Chapter 3.4.2 --- Cloning --- p.34 / Chapter 3.4.3 --- Transformation --- p.36 / Chapter 3.4.4 --- Screening --- p.36 / Chapter 3.4.4.1 --- Restriction enzyme digestion of plasmid DNA --- p.36 / Chapter 3.4.4.2 --- PCR Screening --- p.38 / Chapter 3.5 --- DNA sequencing --- p.38 / Chapter 3.5.1 --- Preparation of double-stranded template --- p.38 / Chapter 3.5.2 --- Sequencing reaction using double-stranded templates --- p.39 / Chapter 3.5.3 --- Electrophoresis --- p.40 / Chapter 3.5.4 --- Autoradiography and analysis --- p.41 / Chapter 3.6 --- Dot-blot analysis --- p.41 / Chapter 3.6.1 --- Dot-blot --- p.41 / Chapter 3.6.2 --- Probe preparation --- p.42 / Chapter 3.6.3 --- Prehybridization and hybridization --- p.43 / Chapter 3.6.4 --- Autoradiography and Analysis --- p.43 / Chapter 3.7 --- Study on one clone: the gene encoding ubiquitin --- p.44 / Chapter 3.7.1 --- "Design of primers:UBR5A, UBR3A and Polyub5A, polyub3A" --- p.44 / Chapter 3.7.2 --- Isolation of DNA from mycelium and fruit body --- p.46 / Chapter 3.7.3 --- Specific PCR with Polyub primers --- p.46 / Chapter 3.7.4 --- RT-PCR --- p.47 / Chapter 4. --- RESULTS --- p.48 / Chapter 4.1 --- Total RNA isolation --- p.48 / Chapter 4.2 --- RNA AP-PCR --- p.52 / Chapter 4.3 --- PCR reamplification --- p.58 / Chapter 4.4 --- Molecular cloning of RAP products --- p.58 / Chapter 4.5 --- Screening --- p.61 / Chapter 4.6 --- DNA sequencing --- p.65 / Chapter 4.7 --- Sequence analyses --- p.80 / Chapter 4.8 --- Dot blot --- p.84 / Chapter 4.9 --- Further study on pMrG290a --- p.84 / Chapter 5. --- DISCUSSION --- p.92 / Chapter 5.1 --- RNA AP-PCR --- p.92 / Chapter 5.2 --- PCR cloning --- p.94 / Chapter 5.3 --- Nucleotide sequencing --- p.95 / Chapter 5.4 --- Dot blot --- p.96 / Chapter 5.5 --- Effect of polyphenol in RNA or DNA samples to the efficiency of reverse transcription and PCR --- p.98 / Chapter 5.6 --- Ubiquitin and fruit body development --- p.99 / Chapter 5.7 --- "Mitochondrial biogenesis, bioenergetics and fruit body development" --- p.102 / CONCLUSION --- p.105 / REFERENCES --- p.107

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_321618
Date January 1996
ContributorsXie, Weijun., Chinese University of Hong Kong Graduate School. Division of Biology.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, x, 122 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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