Return to search

Two partners of the ribosome, EF-Tu and LepA

The translational GTPases elongation factor Tu (EF-Tu) and LepA modulate the dynamics of tRNA on the ribosome. EF-Tu facilitates the delivery of aminoacyl-tRNA (aa-tRNA) to the translating ribosome and LepA catalyzes the retro-translocation of tRNA•mRNA from the E- and P-sites of the ribosome back to the P- and A-sites. Although an increasing body of structural and biochemical information is available, little is known about the functional cycle of LepA during retro-translocation, the kinetics of EF-Tu dissociation from the ribosome and the rate of EF-Tu conformational change during aa-tRNA delivery. This thesis reports the successful construction and biochemical characterisation of a mutant form of EF-Tu from Escherichia coli ideal for the specific incorporation of fluorescent labels, enabling measurements pivotal for uncovering the rate of EF-Tu conformational change and dissociation from the ribosome. Furthermore, to determine structural components critical for LepA’s function, mutant versions of the protein were constructed and biochemically characterised. / xii, 127 leaves : ill. (some col.) ; 29 cm

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:ALU.w.uleth.ca/dspace#10133/2515
Date January 2009
Creatorsde Laurentiis, Evelina Ines, University of Lethbridge. Faculty of Arts and Science
ContributorsWieden, Hans-Joachim
PublisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry, c2009, Arts and Science, Department of Chemistry and Biochemistry
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
Languageen_US
Detected LanguageEnglish
TypeThesis
RelationThesis (University of Lethbridge. Faculty of Arts and Science)

Page generated in 0.002 seconds