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Previous issue date: 2017-01-19 / The present work consists in the specific delimitation study of Croton muscicapa M?ll. Arg. and Croton longibracteatus Mart. Gord. & de Luna, belonging to Croton L., one of the biggest genus of Euphorbiaceae. It was analyzed the differentiation and the relations between these two species and associated morphotypes occurring along Espinha?o Range and adjacent areas in Brazil. For this purpose, samples of two species from herbarium were previously analyzed and 11 populations were collected, which correspond to five morphological groups, being used different methods of analysis. Morphotypes were tested by traditional uni and multivariated morphometrics involving 37 vegetative and floral variables. In addition, analyses of structuring and genetic variability using ISSR markers were realized, selecting the eight most variable primers and obtained 116 polymorphic loci. Foliar anatomy and micromorphology data was obtained from representatives of the 11 populations by optical and scanning electronic microscopy, respectively. Based on analyzed samples, it was observed that vegetative characters are much more variable than reproductive ones and are less taxonomically informative in this group. From the genetic point of view, the populations demonstrated high structuring and low variability. These different lines of evidence confirmed four distinct groups, two corresponding to C. muscicapa and C. longibracteatus, restricted to Chapada Diamantina and adjacent areas in Bahia, Brazil. Two new species are proposed (C. aemulus Barbosa & Carn.-Torres and C. graomogolensis Barbosa & Carn.-Torres), endemic to North of Minas Gerais. These species can be separated principally by limb indumentum, quantity and length of glands in the limb margin, length of pistillate and staminate flowers and amount of stamens. Croton aemulus corresponds to a natural hybrid, confirmed by the genetic pool shared between C. longibracteatus and C. graomogolensis. It is presented a recircumscription for C. muscicapa and C. longibracteatus, besides descriptions, key for identification, illustrations, photos, distribution maps and general comments for all species. The four species share anatomical and micromorphological characters, however, differs by the position of stamens, petiole surface, length of glands in the limb margin and quantity of secondary compounds. The two new species are considered as endangered, according to IUCN criteria. / O presente trabalho consiste no estudo de delimita??o espec?fica de Croton muscicapa M?ll. Arg. e Croton longibracteatus Mart. Gord. & de Luna, pertencentes ao g?nero Croton L., um dos maiores de Euphorbiaceae. Foi analisada a diferencia??o e as rela??es entre essas duas esp?cies e morfotipos associados ocorrentes ao longo da Cadeia do Espinha?o e ?reas adjacentes no Brasil. Para isso, foram previamente analisadas amostras das duas esp?cies em herb?rio e coletadas 11 popula??es que correspondem a cinco grupos morfol?gicos, sendo aplicados diferentes m?todos de an?lise. Os morfotipos foram inicialmente testados atrav?s de morfometria tradicional uni e multivariada, envolvendo 37 vari?veis vegetativas e florais. Tamb?m foram realizadas an?lises de estrutura??o e variabilidade gen?tica a partir de marcadores moleculares ISSR, e sendo selecionados os oito primers mais vari?veis e obtidas 116 loci polim?rficos. Dados de anatomia e micromorfologia foliar tamb?m foram obtidos para representantes das 11 popula??es, atrav?s de microscopia ?ptica e microscopia elet?nica de varredura, respectivamente. A partir das amostras analisadas, observou-se que os caracteres vegetativos s?o muito mais vari?veis que os reprodutivos e menos informativos para fins taxon?micos nesse grupo. Do ponto de vista gen?tico, as popula??es demonstraram alta estrutura??o e baixa variabilidade. Atrav?s dessas diferentes linhas de evid?ncia, foram confirmados quatro grupos bastante distintos, dois deles correspondendo a C. muscicapa e C. longibracteatus, restritas ? Chapada Diamantina e ?reas adjacentes, no estado da Bahia, Brasil. Duas novas esp?cies s?o propostas (C. aemulus Barbosa & Carn.-Torres e C. graomogolensis Barbosa & Carn.-Torres), end?micas da regi?o norte de Minas Gerais. Estas esp?cies podem ser separadas especialmente por indumento no limbo, quantidade e comprimento de gl?ndulas na margem do limbo, comprimenro das flores pistiladas e estaminadas e quantidade de estames. Croton aemulus corresponde a um h?brido natural, confirmado pelo pool g?nico compartilhado entre C. longibracteatus e C. graomogolensis. ? apresentada uma recircunscri??o para C. muscicapa e C. longibracteatus, al?m de descri??es, chaves para identifica??o, ilustra??es, fotografias, mapas de distribui??o e coment?rios gerais para todas as esp?cies. Com base nos caracteres anat?micos e micromorfol?gicos, essas quatro esp?cies apresentam alguns caracteres compartilhados, mas diferem pela posi??o dos est?matos, superf?cie do pec?olo, comprimento das gl?ndulas da margem do limbo e a quantidade de compostos secund?rios. As duas novas esp?cies s?o consideradas amea?adas de extin??o, de acordo com os crit?rios da IUCN.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.uefs.br:8080:tede/592 |
| Date | 19 January 2017 |
| Creators | Barbosa, B?rbara La?s Ramos |
| Contributors | Torres, Daniela Santos Carneiro |
| Publisher | Universidade Estadual de Feira de Santana, Mestrado Acad?mico em Recursos Gen?ticos Vegetais, UEFS, Brasil, DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UEFS, instname:Universidade Estadual de Feira de Santana, instacron:UEFS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 8146646481622241874, 600, 600, 600, 5026123383450589282, -3439178843068202161 |
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